Kleine-Tebbe J, Galleani M, Jeep S, Pilz B, Baisch A, Kunkel G
Dept. of Clinical Immunology and Asthma OPD, University Hospital Rudolf Virchow, Free University, Berlin, Germany.
Allergy. 1992 Dec;47(6):618-23. doi: 10.1111/j.1398-9995.1992.tb02384.x.
Histamine release (HR) studies were performed in 40 birch pollen-allergic patients (positive case history, positive SPT, positive birch pollen-specific serum IgE: RAST > or = 3) with (n = 20, A) and without (n = 20, B) fruit hypersensitivity, and 10 nonatopic volunteers (C). Several fruit allergens were used and characterized by protein determination and immunoblot techniques. Dose-dependent HR (apple peel = apple pulp > peach = cherry) was demonstrated in both allergic groups, but to a higher extent in patients with fruit allergy (P < 0.01). Increased basophil sensitivity to birch pollen was found in the group with fruit allergy (P < 0.001). Strong correlations between the mediator response induced by several fruits indicate common allergens within the extracts. We conclude that fruit-related symptoms require not only high specific serum IgE, but a strong cellular sensitization to birch pollen allergens together with an increased cellular reactivity to fruit allergens.
对40名桦树花粉过敏患者（有阳性病史、阳性皮肤点刺试验、阳性桦树花粉特异性血清IgE：放射性过敏原吸附试验≥3）进行了组胺释放（HR）研究，其中20名有水果过敏（A组），20名无水果过敏（B组），并选取了10名非特应性志愿者作为对照（C组）。使用了几种水果过敏原，并通过蛋白质测定和免疫印迹技术对其进行了鉴定。在两个过敏组中均证实了剂量依赖性HR（苹果皮=苹果果肉＞桃子=樱桃），但在水果过敏患者中程度更高（P＜0.01）。在水果过敏组中发现嗜碱性粒细胞对桦树花粉的敏感性增加（P＜0.001）。几种水果诱导的介质反应之间存在强相关性，表明提取物中存在共同过敏原。我们得出结论，与水果相关的症状不仅需要高特异性血清IgE，还需要对桦树花粉过敏原的强烈细胞致敏以及对水果过敏原的细胞反应性增加。
