Kim Chang-Bae, Shashikant Cooduvalli S, Sumiyama Kenta, Wang Wayne C H, Amemiya Chris T, Ruddle Frank H
Department of Molecular, Cellular, and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520, USA.
J Struct Funct Genomics. 2003;3(1-4):195-9.
The non-coding intergenic regions of Hox genes are remarkably conserved among mammals. To determine the usefulness of this sequence for phylogenetic comparisons, we sequenced an 800-bp fragment of the Hoxc9-Hoxc8 intergenic region from several species belonging to different mammalian clades. Results obtained from the phylogenetic analysis are congruent with currently accepted mammalian phylogeny. Additionally, we found a TC mini satellite repeat polymorphism unique to felines. This polymorphism may serve as a useful marker to differentiate between mammalian species or as a genetic marker in feline matings. This study demonstrates usefulness of a comparative approach employing non-coding regions of Hox gene complexes.
Hox基因的非编码基因间区域在哺乳动物中具有显著的保守性。为了确定该序列在系统发育比较中的有用性，我们对来自不同哺乳动物类群的几个物种的Hoxc9 - Hoxc8基因间区域的一个800碱基对片段进行了测序。系统发育分析的结果与目前公认的哺乳动物系统发育一致。此外，我们发现了一种猫科动物特有的TC小卫星重复多态性。这种多态性可作为区分哺乳动物物种的有用标记或作为猫科动物交配中的遗传标记。这项研究证明了采用Hox基因复合体非编码区域的比较方法的有用性。
