使用钛宝石再生放大器对活体大脑进行深度达1000微米的双光子成像。
Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier.
作者信息
Theer Patrick, Hasan Mazahir T, Denk Winfried
机构信息
Max-Planck Institute for Medical Research, Johnstrasse 29, D-69120 Heidelberg, Germany.
出版信息
Opt Lett. 2003 Jun 15;28(12):1022-4. doi: 10.1364/ol.28.001022.
PMID:12836766
Abstract
It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence.
摘要
结果表明，通过使用光放大飞秒脉冲，可以在小鼠新皮质几乎整个灰质区域获得双光子荧光图像。所实现的成像深度接近由离焦荧光激发所设定的理论极限。
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