Theer Patrick, Hasan Mazahir T, Denk Winfried
Max-Planck Institute for Medical Research, Johnstrasse 29, D-69120 Heidelberg, Germany.
Opt Lett. 2003 Jun 15;28(12):1022-4. doi: 10.1364/ol.28.001022.
It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence.
结果表明，通过使用光放大飞秒脉冲，可以在小鼠新皮质几乎整个灰质区域获得双光子荧光图像。所实现的成像深度接近由离焦荧光激发所设定的理论极限。
