Yang L J
Stomatological College, Fourth Military Medical University, Xian.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1992 Mar;27(2):102-3, 128.
A cell strain stably secreting monoclonal antibody against BMP was obtained by hybridoma technique. The monoclonal antibody specifically reacted with the cells of human bone and tooth germs on paraffin embedded tissue sections. Immunohistochemical staining shows that BMP is distributed along collagen fibres in normal bone, also exist in osteoid tissue of new bone, in osteoblasts and in the cells of bone marrow. BMP may be found in human tooth germs such as in predentin, in the cells of outer and inner enamel epithelium, in the cells of dental sac generating alveolar bone. The results demonstrate that generation and growth of human bone and development of tooth germs have relation to BMP. The results at the molecular level prove that some antigenic determinants of human and bovine BMP are the same. BMP function activity inhibition test suggests that this antibody may block the function group of BMP. The ability of the monoclonal antibody to detect antigen and to inhibit generation of new bone makes it potentially useful in purification of BMP and in treating osteosarcoma and other bone tumors.
通过杂交瘤技术获得了一种稳定分泌抗骨形态发生蛋白（BMP）单克隆抗体的细胞株。该单克隆抗体与石蜡包埋组织切片上的人骨和牙胚细胞发生特异性反应。免疫组织化学染色显示，BMP在正常骨中沿胶原纤维分布，也存在于新骨的类骨质组织、成骨细胞和骨髓细胞中。BMP可在人牙胚中发现，如前期牙本质、外釉上皮和内釉上皮细胞以及形成牙槽骨的牙囊细胞中。结果表明，人骨的生成和生长以及牙胚的发育与BMP有关。分子水平的结果证明，人和牛BMP的一些抗原决定簇是相同的。BMP功能活性抑制试验表明，该抗体可能阻断BMP的功能基团。该单克隆抗体检测抗原和抑制新骨生成的能力使其在BMP的纯化以及骨肉瘤和其他骨肿瘤的治疗中具有潜在的应用价值。
