Yang L J, Jin Y
Department of Oral Pathology, Stomatological College, Fourth Military Medical University, Xi'an, P.R. China.
Clin Orthop Relat Res. 1990 Aug(257):249-56.
Localization of bone morphogenetic protein (BMP) in human tissues and cells is important for investigating the mechanism of bone induction. A stable cell line secreting monoclonal antibody against bovine BMP (bBMP-McAb) was obtained by the hybridoma technique. The result of immunohistochemical staining (ABC method) showed that BMP is distributed along collagen fibers of normal bone, in periosteal cells, and in mesenchymal cells of marrow stroma. Little BMP can be found in bone cells of lamellar bone or in calcified bone matrix. BMP may be abundant in human tooth anlagen such as predentin, cells of the outer and inner enamel epithelium, and cells of dental sac generating bone. BMP is found in the cytoplasm of tumor cells of osteosarcoma and chondrosarcoma. Immunohistochemical staining showed that BMP plays a role in bone fracture healing. The ability of BMP-McAb to detect BMP and to inhibit the generation of new bone also makes it potentially useful in diagnosing, treating, and providing a prognosis for osteosarcoma and other bone diseases.
骨形态发生蛋白（BMP）在人体组织和细胞中的定位对于研究骨诱导机制很重要。通过杂交瘤技术获得了一种稳定分泌抗牛BMP单克隆抗体（bBMP - McAb）的细胞系。免疫组织化学染色结果（ABC法）显示，BMP沿正常骨的胶原纤维分布于骨膜细胞和骨髓基质的间充质细胞中。在板层骨的骨细胞或钙化骨基质中几乎找不到BMP。BMP在人类牙胚中可能含量丰富，如前期牙本质、内釉上皮和外釉上皮细胞以及产生骨的牙囊细胞。在骨肉瘤和软骨肉瘤的肿瘤细胞胞质中发现有BMP。免疫组织化学染色表明BMP在骨折愈合中起作用。BMP - McAb检测BMP以及抑制新骨生成的能力也使其在骨肉瘤和其他骨疾病的诊断、治疗及预后判断方面具有潜在用途。
