Nakamura M, Kobayashi M, Hirano K, Kobayashi K, Hoshino T, Awaya S
Department of Ophthalmology, Nagoya University School of Medicine, Japan.
Jpn J Ophthalmol. 1992;36(4):458-64.
An experimental model for the age-related changes in the extracellular matrix of the human cornea was developed. Human infant corneal stroma in which no long-spacing collagen occurred naturally was treated with 20 mM adenosine triphosphate (ATP). The ATP-treated cornea was observed and compared with nontreated control specimens by electron microscopy. Numerous 100 nm periodic fibrils which resembled long-spacing collagen were formed by the treatment. These experimentally formed fibrils appeared to attract and aggregate the collagen fibrils with D-periodicity, which indicated a connection between type VI and type I collagen. By ruthenium red staining, the cross-bands of the 100 nm periodic fibrils were positively stained, indicating that glycosaminoglycans or proteoglycans were involved in the formation of these fibrils.
建立了一个关于人角膜细胞外基质年龄相关变化的实验模型。对天然不存在长间距胶原的人类婴儿角膜基质用20 mM三磷酸腺苷（ATP）进行处理。通过电子显微镜观察经ATP处理的角膜，并与未处理的对照标本进行比较。处理后形成了许多类似于长间距胶原的100 nm周期性纤维。这些实验形成的纤维似乎吸引并聚集了具有D周期的胶原纤维，这表明VI型和I型胶原之间存在联系。通过钌红染色，100 nm周期性纤维的交叉带被阳性染色，表明糖胺聚糖或蛋白聚糖参与了这些纤维的形成。
