Komatsu Y, Koizumi M, Sekiguchi A, Ohtsuka E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nucleic Acids Symp Ser. 1992(27):43-4.
The catalytic domain in the minus strand of the satellite RNA of tobacco ringspot virus (sTobRV(-)) assumes a hairpin-like secondary structure. This ribozyme catalyzes a cross-ligation reaction between substrate RNAs of different lengths. We constructed ribozymes to probe the activities of ligation and RNA fragment exchange.
烟草环斑病毒卫星RNA负链(sTobRV(-))中的催化结构域呈现出类似发夹的二级结构。这种核酶催化不同长度底物RNA之间的交叉连接反应。我们构建了核酶以探究连接和RNA片段交换的活性。