Komatsu Y, Koizumi M, Sekiguchi A, Ohtsuka E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nucleic Acids Res. 1993 Jan 25;21(2):185-90. doi: 10.1093/nar/21.2.185.
The negative strand of the satellite RNA of tobacco ringspot virus (sTobRV(-)) contains a hairpin catalytic domain that shows self-cleavage and self-ligation activities in the presence of magnesium ions. We describe here that the minimal catalytic domain can catalyze a cross-ligation reaction between two kinds of substrates in trans. The cross-ligated product increased when the reaction temperature was decreased during the reaction from 37 degrees C to 4 degrees C. A two-stranded hairpin ribozyme, divided into two fragments between G45 and U46 in a hairpin loop, showed higher ligation activity than the nondivided ribozyme. The two stranded ribozyme also catalyzed an exchange reaction of the 3'-portion of the cleavage site.
烟草环斑病毒卫星RNA的负链（sTobRV(-)）含有一个发夹催化结构域，该结构域在镁离子存在下表现出自我切割和自我连接活性。我们在此描述，最小催化结构域可以催化两种底物之间的反式交叉连接反应。当反应温度在反应过程中从37℃降至4℃时，交叉连接产物增加。一种双链发夹核酶，在发夹环的G45和U46之间分为两个片段，其连接活性高于未分割的核酶。双链核酶还催化了切割位点3'部分的交换反应。
