Karlson Dale T, Fujino Takeshi, Kimura Satoshi, Baba Kei'ichi, Itoh Takao, Ashworth Edward N
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-1165, USA.
Tree Physiol. 2003 Aug;23(11):759-67. doi: 10.1093/treephys/23.11.759.
Dehydrins are proteins associated with conditions affecting the water status of plant cells, such as drought, salinity, freezing and seed maturation. Although the function of dehydrins remains unknown, it is hypothesized that they stabilize membranes and macromolecules during cellular dehydration. Red-osier dogwood (Cornus sericea L.), an extremely freeze-tolerant woody plant, accumulates dehydrin-like proteins during cold acclimation and the presence of these proteins is correlated with increased freeze tolerance (Karlson 2001, Sarnighausen et al. 2002, Karlson et al. 2003). Our objective was to determine the location of dehydrins in cold-acclimated C. sericea stems in an effort to provide insight into their potential role in the freeze tolerance of this extremely cold hardy species. Abundant labeling was observed in the nucleus and cytoplasm of cold-acclimated C. sericea stem cells. In addition, labeling was observed in association with plasmodesmata of cold-acclimated vascular cambium cells. The unique association of dehydrin-like proteins with plasmodesmata has not been reported previously.
脱水素是与影响植物细胞水分状况的条件相关的蛋白质，如干旱、盐度、冷冻和种子成熟。尽管脱水素的功能尚不清楚，但据推测，它们在细胞脱水过程中能稳定细胞膜和大分子。红枝山茱萸（Cornus sericea L.）是一种极其耐寒的木本植物，在低温驯化过程中会积累类似脱水素的蛋白质，这些蛋白质的存在与耐寒性的提高相关（卡尔森，2001年；萨尼豪森等人，2002年；卡尔森等人，2003年）。我们的目标是确定脱水素在低温驯化的红枝山茱萸茎中的位置，以便深入了解它们在这种极其耐寒的物种的耐寒性中可能发挥的作用。在低温驯化的红枝山茱萸茎细胞的细胞核和细胞质中观察到大量标记。此外，在低温驯化的维管形成层细胞的胞间连丝处也观察到标记。类似脱水素的蛋白质与胞间连丝的独特关联此前尚未见报道。
