三个脱水素基因的异源表达赋予了[具体物种]对非生物胁迫的耐受性。（原文此处“in”后缺少具体物种信息）

Heterologous Expression of Three Dehydrin Genes Confers Abiotic Stress Tolerance in .

作者信息

Cui Hongwei, Wang Yang, Yu Tingqiao, Chen Shaoliang, Chen Yuzhen, Lu Cunfu

机构信息

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.

College of Life Science, Pecking University, Beijing 100083, China.

出版信息

Plants (Basel). 2020 Feb 5;9(2):193. doi: 10.3390/plants9020193.

, a xerophyte plant that belongs to the family Leguminosae, adapts to extremely arid, hot, and cold environments, making it an excellent woody plant to study the molecular mechanisms underlying abiotic stress tolerance. Three dehydrin genes, , , and were cloned from abiotic stress treated seedlings. Cytomembrane-located AmDHN200, nucleus-located AmDHN154, and cytoplasm and nucleus-located AmDHN132 were characterized by constitutive overexpression of their genes in . Overexpression of AmDHN132, AmDHN154, and AmDHN200 in transgenic Arabidopsis improved salt, osmotic, and cold tolerances, with AmDHN132 having the largest effect, whereas the growth of transformed plants is not negatively affected. These results indicate that AmDHNs contribute to the abiotic stress tolerance of and that genes function differently in response to abiotic stresses. Furthermore, they have the potential to be used in the genetic engineering of stress tolerance in higher plants.

作为一种属于豆科的旱生植物，适应极端干旱、炎热和寒冷的环境，使其成为研究非生物胁迫耐受性分子机制的优良木本植物。从经非生物胁迫处理的幼苗中克隆了三个脱水素基因，即、和。细胞膜定位的AmDHN200、细胞核定位的AmDHN154以及细胞质和细胞核定位的AmDHN132通过在其基因的组成型过表达来表征。在转基因拟南芥中过表达AmDHN132、AmDHN154和AmDHN200提高了盐、渗透和耐寒性，其中AmDHN132的效果最大，而转化植物的生长没有受到负面影响。这些结果表明，AmDHNs有助于的非生物胁迫耐受性，并且基因在响应非生物胁迫时功能不同。此外，它们有潜力用于高等植物抗逆性的基因工程。