Rontani Jean-François, Mouzdahir Abdelkrim, Michotey Valerie, Caumette Pierre, Bonin Patricia
Laboratoire d'Océanographie et de Biogéochimie (UMR 6535), Centre d'Océanologie de Marseille-OSU, Campus de Luminy, case 901, 13288 Marseille, France.
Appl Environ Microbiol. 2003 Jul;69(7):4167-76. doi: 10.1128/AEM.69.7.4167-4176.2003.
This paper describes the production of 5,9,13-trimethyltetradeca-4E,8E,12-trienyl-5,9,13-trimethyltetradeca-4E,8E,12-trienoate during the aerobic degradation of squalene by a Marinobacter strain, 2Asq64, isolated from the marine environment. A pathway involving initial cleavage of the C(10)-C(11) or C(14)-C(15) double bonds of the squalene molecule is proposed to explain the formation of this polyunsaturated isoprenoid wax ester. The isoprenoid wax ester content reached 1.1% of the degraded squalene at the mid-exponential growth phase and then decreased during the stationary phase. The wax ester content increased by approximately threefold in N-limited cultures, in which the ammonium concentration corresponds to conditions often found in marine sediments. This suggests that the bacterial formation of isoprenoid wax esters might be favored in such environments. The bacterial strain is then characterized as a member of a new species, for which we propose the name Marinobacter squalenivorans sp. nov.
本文描述了从海洋环境中分离出的一株海杆菌菌株2Asq64在需氧降解角鲨烯过程中5,9,13-三甲基十四碳-4E,8E,12-三烯基-5,9,13-三甲基十四碳-4E,8E,12-三烯酸酯的产生情况。提出了一条涉及角鲨烯分子中C(10)-C(11)或C(14)-C(15)双键初始断裂的途径来解释这种多不饱和类异戊二烯蜡酯的形成。类异戊二烯蜡酯含量在指数生长中期达到降解角鲨烯的1.1%，然后在稳定期下降。在氮限制培养物中蜡酯含量增加了约三倍，其中铵浓度对应于海洋沉积物中常见的条件。这表明在这样的环境中细菌形成类异戊二烯蜡酯可能更有利。该细菌菌株随后被鉴定为一个新物种的成员，我们为此提议将其命名为食角鲨烯海杆菌新种（Marinobacter squalenivorans sp. nov.）。
