Moustafa Mohamed E, Kumaraswamy Easwari, Zhong Nianxin, Rao Mahadev, Carlson Bradley A, Hatfield Dolph L
Section on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
J Nutr. 2003 Jul;133(7 Suppl):2494S-2496S. doi: 10.1093/jn/133.7.2494S.
Two model systems for examining the role of selenoproteins in health are discussed. One utilizes transgenic mice that carry mutant selenocysteine (Sec) tRNA transgenes that result in the reduction of selenoprotein expression in a protein- and tissue-specific manner. The other utilizes loxP-Cre technology to selectively remove the Sec tRNA gene in mammary epithelium that results in the reduction of only certain selenoproteins in this tissue. Both approaches provide important tools for examining the role of selenoproteins in health.
本文讨论了两种用于研究硒蛋白在健康中的作用的模型系统。一种利用携带突变硒代半胱氨酸（Sec）tRNA转基因的转基因小鼠，这些转基因会以蛋白质和组织特异性方式导致硒蛋白表达降低。另一种利用loxP-Cre技术选择性地去除乳腺上皮中的Sec tRNA基因，从而导致该组织中仅某些硒蛋白的表达降低。这两种方法都为研究硒蛋白在健康中的作用提供了重要工具。
