Li J, Chen D, Tian Y
Department of Surgery, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
J Tongji Med Univ. 1999;19(3):226-9. doi: 10.1007/BF02887741.
Fifty-six SD rats were randomly divided into the normal control group and the operation group. The operating group was subdivided into six groups on the basis of killing time (the 12th h, the first day, 3rd day, first week, 2nd week and 4th week) after vagotomy (VG). The pancreatic tissues were taken for HE and Feulgen staining. The DNA content of pancreatic exocrine cells was determined by a domestically-fabricated computer image analyzing system. In the control group, on the first day or in the first week after VG, the pancreatic samples were taken for transmission electron microscopic examination. The DNA content of pancreatic exocrinal cells was decreased from 1 to 3 days after VG. The secretion was found to be in inhibitory state and One week later, it gradually restored. The results indicated that the proliferation and the function of the SD rat's pancreatic exocrinal cells were prohibited at initial stage after VG, which might be concerned, at least in part, with dominance and nutrition of vagus.
56只SD大鼠被随机分为正常对照组和手术组。手术组根据迷走神经切断术（VG）后处死时间（第12小时、第1天、第3天、第1周、第2周和第4周）再细分为6组。取胰腺组织进行苏木精-伊红（HE）染色和福尔根染色。用国产计算机图像分析系统测定胰腺外分泌细胞的DNA含量。对照组在迷走神经切断术后第1天或第1周取胰腺样本进行透射电镜检查。迷走神经切断术后1至3天，胰腺外分泌细胞的DNA含量降低。发现分泌处于抑制状态，1周后逐渐恢复。结果表明，迷走神经切断术后初期，SD大鼠胰腺外分泌细胞的增殖和功能受到抑制，这可能至少部分与迷走神经的支配和营养有关。
