Thambiah G, Whitworth J, Hommel M, Devaney E
Liverpool School of Tropical Medicine, U.K.
Trop Med Parasitol. 1992 Dec;43(4):271-6.
A PEG-ELISA was used to demonstrate parasite specific immune complexes in a significant proportion (25/26) of Onchocerca volvulus infection sera from Sierra Leone. The parasite antigen was detected using a peroxidase-conjugated rabbit serum raised to the bovine parasite O. gibsoni. Controls including European control serum, endemic control serum and Rh+ sera gave consistently low readings. Characterization of the parasite component in the immune complexes by Western blotting demonstrated a heat stable antigen of M(r) 46,000. This antigen was not present in the circulating immune complexes (CIC) prepared from patients with Wuchereria bancrofti infection, but a cross-reactive molecule of the same size was weakly recognized in the CIC of Loa loa and Mansonella perstans infected patients. No association between the level of parasite specific CIC and clinical disease was observed in the O. volvulus patients.
采用聚乙二醇（PEG）-酶联免疫吸附测定法（ELISA），在来自塞拉利昂的大量（25/26）盘尾丝虫感染血清中证实了寄生虫特异性免疫复合物的存在。使用针对牛寄生虫吉氏盘尾丝虫制备的过氧化物酶偶联兔血清检测寄生虫抗原。包括欧洲对照血清、地方性对照血清和Rh+血清在内的对照血清读数始终较低。通过蛋白质免疫印迹法对免疫复合物中的寄生虫成分进行表征，显示出一种分子量为46,000的热稳定抗原。该抗原不存在于班氏吴策线虫感染患者制备的循环免疫复合物（CIC）中，但在罗阿丝虫和常现曼森线虫感染患者的CIC中可微弱识别出相同大小的交叉反应分子。在盘尾丝虫病患者中未观察到寄生虫特异性CIC水平与临床疾病之间的关联。
