绿原酸对羟基自由基的影响。
Effect of chlorogenic acid on hydroxyl radical.
作者信息
Zang Lun-Yi, Cosma Greg, Gardner Henry, Castranova Vince, Vallyathan Val
机构信息
Exposure Assessment Branch and Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA.
出版信息
Mol Cell Biochem. 2003 May;247(1-2):205-10. doi: 10.1023/a:1024103428348.
Chlorogenic acid (CGA) is considered to act as an antioxidant. However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (*OH). We found that CGA scavenges *OH in a dose-dependent manner. The kinetic parameters, IC50 and Vmax, for CGA scavenging of *OH were 110 and 1.27 microM/sec, respectively. The rate constant for the scavenging of *OH by CGA was 7.73 x 10(9) M(-1) sec(-1). Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on *OH.
绿原酸（CGA）被认为具有抗氧化作用。然而，CGA对特定自由基种类的抑制作用尚未得到充分了解。利用电子自旋共振（ESR）结合自旋捕获技术来检测自由基。5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）用作自旋捕获试剂，而芬顿反应用作羟基自由基（OH）的来源。我们发现CGA以剂量依赖的方式清除OH。CGA清除OH的动力学参数IC50和Vmax分别为110和1.27微摩尔/秒。CGA清除OH的速率常数为7.73×10⁹ M⁻¹秒⁻¹。我们的研究表明，CGA的抗氧化特性可能涉及CGA对*OH的直接清除作用。
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