Barrou B, Legac E, Bitker M O, Jacobs C, Chatelain C, Debré P, Autran B
Service d'Urologie, Hôpital de la Pitié-Salpêtrière, Paris.
Presse Med. 1992 Dec 2;21(41):1989-90.
A minor subset of CD4+ T-cells (10 +/- 4 percent) that does not express the CD7 antigen is detectable in normal individuals. These CD4+CD7- T-cells have lower proliferative capacities than autologous CD4+CD7- T-cells. We observed an expansion of CD4+CD7- peripheral T-cells in 45 kidney transplant recipients. The CD4+CD7- TL expansion (23 +/- 20 percent was observed in both short term (< 6 months, n = 20) and long term transplanted patients (> 3 years, n = 25). These results significantly differed from those observed in patients on dialysis (10 +/- 4 percent, P < 0.002). The percentages of CD4+CD7- TL in kidney recipients are significantly correlated with the percentages of CD4+CD57+ and CD8+CD57+ T-cells subsets. A three-color immunofluorescence analysis indicated that 75 percent of the CD4+CD7- TL express the CD57 molecule contrasting with those in normal individuals (0.3 percent). Purified CD4+CD7- T-cells from kidney recipients have lower proliferative responses after PHA, CD2 and CD3 stimulations than autologous CD4+CD7+ TL. The proliferative response is partially restored by the addition of anti-CD28 moAb or IL2. We have highlighted, in kidney recipients with good graft function, an expansion of CD4+CD7- T lymphocytes which could be associated with CD4+ T-cell anergy and tolerance.
在正常个体中可检测到一小部分不表达CD7抗原的CD4 + T细胞（10±4%）。这些CD4 + CD7 - T细胞的增殖能力低于自体CD4 + CD7 + T细胞。我们观察到45例肾移植受者中CD4 + CD7 - 外周T细胞出现扩增。在短期（<6个月，n = 20）和长期移植患者（>3年，n = 25）中均观察到CD4 + CD7 - T淋巴细胞扩增（23±20%）。这些结果与透析患者中观察到的结果（10±4%，P < 0.002）有显著差异。肾移植受者中CD4 + CD7 - T淋巴细胞的百分比与CD4 + CD57 +和CD8 + CD57 + T细胞亚群的百分比显著相关。三色免疫荧光分析表明，75%的CD4 + CD7 - T淋巴细胞表达CD57分子，这与正常个体中的情况（0.3%）形成对比。肾移植受者纯化的CD4 + CD7 - T细胞在PHA、CD2和CD3刺激后的增殖反应低于自体CD4 + CD7 + T淋巴细胞。添加抗CD28单克隆抗体或IL2后，增殖反应部分恢复。我们强调，在移植肾功能良好的肾移植受者中，CD4 + CD7 - T淋巴细胞出现扩增，这可能与CD4 + T细胞无反应性和耐受性有关。
