Yoshida Y, Hasegawa R, Takaba K, Imaida K, Ito N
First Department of Pathology, Nagoya City University Medical School, Japan.
Teratog Carcinog Mutagen. 1992;12(5):197-201. doi: 10.1002/tcm.1770120502.
The combination effects of bleomycin with N-nitrosoheptamethyleneimine (NHMI) or dihydroxy-di-N-propylnitrosamine (DHPN) on pulmonary carcinogenesis were investigated. Male F344 rats were given NHMI (20 or 40 ppm) or DHPN (200 ppm) in the drinking water and intraperitoneally injected with bleomycin (1 mg/kg) once a week for 18 weeks and then killed at week 24. Many rats treated with NHMI died before the termination of the experiment due to toxicity or development of advanced esophageal carcinomas, considered to be the main cause of death. Detailed histological examination performed on rats killed at week 24 revealed no statistically significant effects of bleomycin on NHMI or DHPN induction of neoplastic lesions in the lung or esophagus, although pulmonary carcinomas were only found in two rats treated with NHMI plus bleomycin. Under the present experimental conditions, NHMI exerted stronger carcinogenic activity in the esophagus than in the lung, and no obvious modifying effects of simultaneously administered bleomycin were evident on NHMI- or DHPN-induced pulmonary carcinogenesis.
研究了博来霉素与N-亚硝基庚亚甲基亚胺（NHMI）或二羟基二-N-丙基亚硝胺（DHPN）联合作用对肺癌发生的影响。雄性F344大鼠饮用含NHMI（20或40 ppm）或DHPN（200 ppm）的水，并每周腹腔注射一次博来霉素（1 mg/kg），持续18周，然后在第24周处死。许多接受NHMI治疗的大鼠在实验结束前因毒性或晚期食管癌的发展而死亡，这被认为是主要死因。对在第24周处死的大鼠进行的详细组织学检查显示，博来霉素对NHMI或DHPN诱导的肺或食管肿瘤性病变没有统计学上的显著影响，尽管仅在两只接受NHMI加博来霉素治疗的大鼠中发现了肺癌。在目前的实验条件下，NHMI在食管中的致癌活性比在肺中更强，同时给予的博来霉素对NHMI或DHPN诱导的肺癌发生没有明显的修饰作用。
