Rao M S, Reddy J K
J Natl Cancer Inst. 1980 Oct;65(4):835-40. doi: 10.1093/jnci/65.4.835.
The carcinogenic effect of 2,2'-dihydroxy-di-n-propylnitrosamine (DHPN) was examined in the primitive primate, tree shrew (Tupaia glis). DHPN was administered at a dose of 250 mg/kg body weight sc once a week for 80 weeks. Between 65 and 102 weeks, 8 of 9 males given DHPN (89%) and 11 of 14 females given DHPN (78%) developed pulmonary adenomas. In 2 DHPN-treated males, in addition to adenomas, bronchioalveolar carcinomas were observed. Transmission electron microscopic examination of pulmonary adenomas from 4 DHPN-treated animals showed that Clara cells were the main components of these tumors. In addition to pulmonary tumors, 9% of the DHPN-treated animals developed squamous cell carcinomas of the skin and hepatocellular carcinomas. None of the 6 controls, which received olive oil alone, developed any tumors.
在原始灵长类动物树鼩（Tupaia glis）中检测了2,2'-二羟基二正丙基亚硝胺（DHPN）的致癌作用。以250mg/kg体重的剂量皮下注射DHPN，每周一次，共80周。在65至102周之间，给予DHPN的9只雄性树鼩中有8只（89%）以及给予DHPN的14只雌性树鼩中有11只（78%）发生了肺腺瘤。在2只接受DHPN治疗的雄性树鼩中，除腺瘤外，还观察到细支气管肺泡癌。对4只接受DHPN治疗动物的肺腺瘤进行透射电子显微镜检查显示，克拉拉细胞是这些肿瘤的主要成分。除肺部肿瘤外，9%接受DHPN治疗的动物发生了皮肤鳞状细胞癌和肝细胞癌。6只仅接受橄榄油的对照动物均未发生任何肿瘤。
