Ogata Satoshi, Florese Ruth Huab, Nagano-Fujii Motoko, Hidajat Rachmat, Deng Lin, Ku Yonson, Yoon Seitetsu, Saito Takafumi, Kawata Sumio, Hotta Hak
Division of Microbiology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
J Clin Microbiol. 2003 Jul;41(7):2835-41. doi: 10.1128/JCM.41.7.2835-2841.2003.
The NS3 protein of hepatitis C virus subtype 1b (HCV-1b) isolates obtained from 89 patients with hepatocellular carcinoma (HCC) and 78 patients without HCC were analyzed. On the basis of the secondary structure of the amino-terminal 120 residues of NS3, HCV-1b isolates were classified into group A, group B, and an indeterminate group, each of which was further divided into a number of subgroups, such as A1-1, A1-2, A2-1, A2-2, B1-1, B1-2, B2-1, B2-2, C-1, C-2, and C-3. HCV-1b isolates of subgroup B1-1 were found in 53 (59.6%) of 89 patients with HCC and 19 (24.4%) of 78 patients without HCC, with the difference between the two patient groups being statistically significant (P < 0.00001). Although the number of isolates was small, subgroup B2-1 was also highly associated with HCC, with all five isolates in that subgroup being found in patients with HCC (P < 0.05). On the other hand, HCV-1b isolates of subgroup A1-1 were associated only weakly with HCC; they were found in 6 (6.7%) of 89 patients with HCC and in 25 (32.1%) of 78 patients without HCC, with the difference between the two patient groups being statistically significant (P < 0.0001). The other subgroups, such as A1-2, A2-1, B1-2, C-1, C-2, and C-3, were moderately associated with HCC; their distribution patterns among patients with HCC did not differ significantly from those among patients without HCC. Taken together, our results suggest that HCV-1b isolates of subgroups B1-1 and B2-1 are highly associated with HCC and that this secondary structure analysis may be useful for predicting the relative risk of developing HCC.
对从89例肝细胞癌（HCC）患者和78例非HCC患者中分离出的丙型肝炎病毒1b型（HCV - 1b）毒株的NS3蛋白进行了分析。根据NS3氨基末端120个残基的二级结构，HCV - 1b毒株被分为A组、B组和一个不确定组，每组又进一步分为多个亚组，如A1 - 1、A1 - 2、A2 - 1、A2 - 2、B1 - 1、B1 - 2、B2 - 1、B2 - 2、C - 1、C - 2和C - 3。在89例HCC患者中有53例（59.6%）和78例非HCC患者中有19例（24.4%）检测到B1 - 1亚组的HCV - 1b毒株，两组患者之间的差异具有统计学意义（P < 0.00001）。尽管分离株数量较少，但B2 - 1亚组也与HCC高度相关，该亚组的所有5个分离株均在HCC患者中发现（P < 0.05）。另一方面，A1 - 1亚组的HCV - 1b毒株与HCC的关联较弱；在89例HCC患者中有6例（6.7%）和78例非HCC患者中有25例（32.1%）检测到该毒株，两组患者之间的差异具有统计学意义（P < 0.0001）。其他亚组，如A1 - 2、A2 - 1、B1 - 2、C - 1、C - 2和C - 3，与HCC呈中度相关；它们在HCC患者中的分布模式与非HCC患者中的分布模式没有显著差异。综上所述，我们的结果表明，B1 - 1和B2 - 1亚组的HCV - 1b毒株与HCC高度相关，这种二级结构分析可能有助于预测发生HCC的相对风险。
