Park Chang-Young, Kwak Minjung, Gutierrez Oscar, Graham David Y, Yamaoka Yoshio
Department of Medicine, Veterans Affairs Medical Center, and Baylor College of Medicine, Houston, Texas, USA.
J Clin Microbiol. 2003 Jul;41(7):3336-8. doi: 10.1128/JCM.41.7.3336-3338.2003.
PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.
使用从感染的胃活检标本中分离的DNA对幽门螺杆菌的vacA和cagA基因型进行聚合酶链反应(PCR),其结果与使用培养物中的细菌DNA进行基因分型大致相同。结果不一致与活检中幽门螺杆菌密度低有关。使用活检时发现混合感染的比例更高。