Chattopadhyay Santanu, Patra Rajashree, Ramamurthy T, Chowdhury Abhijit, Santra Amal, Dhali G K, Bhattacharya S K, Berg Douglas E, Nair G Balakrish, Mukhopadhyay Asish K
National Institute of Cholera and Enteric Diseases, Calcutta 700010, India.
J Clin Microbiol. 2004 Jun;42(6):2821-4. doi: 10.1128/JCM.42.6.2821-2824.2004.
We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.
我们开发并评估了一种简单、新颖的多重聚合酶链反应(PCR)检测方法,用于从胃活检标本中快速检测幽门螺杆菌感染以及直接确定vacA和cagA基因分型。该检测方法无需培养菌株或从活检样本中提取DNA。这种多重PCR检测方法对发展中国家的实验室具有特别重要的价值。