Sutherland R M, Durand R E
Curr Top Radiat Res Q. 1976 Jan;11(1):87-139.
Multicell spheroids provide an in vitro tumour model of intermediate complexity between tumours and standard cell cultures. Spheroids of several varieties of normal and tumour cells have been grown and Chinese hamster V79-171b cells have been studied in detail. Spheroids simulate conditions in those solid tumours which contain nodular areas with necrosis at a distance from blood vessels and show a decrease in growth fraction as they increase in size. They contain radiation resistant chronically hypoxic cells which develop spontaneously as the spheroids grow, and exist in a tumour-like environment, yet retain their growth potential. Several assays have been developed or adapted for use in spheroid experiments to study cell properties and responses to treatment. These include many of the techniques used in tumour biology to determine the net response of the tumour, such as volume changes, histology, and net cell survival as well as more direct assays involving separation of subpopulations of cells. Methods were also developed to study in situ repair and repopulation processes after ionizing radiation or other cytotoxic agents.
多细胞球体提供了一种介于肿瘤和标准细胞培养之间的中等复杂性的体外肿瘤模型。已经培养了多种正常细胞和肿瘤细胞的球体，并对中国仓鼠V79-171b细胞进行了详细研究。球体模拟了实体肿瘤中的情况，这些实体肿瘤包含与血管有一定距离且有坏死的结节区域，并且随着尺寸的增加，生长分数会降低。它们含有抗辐射的慢性缺氧细胞，这些细胞在球体生长过程中自发形成，并存在于肿瘤样环境中，但仍保留其生长潜力。已经开发或改编了几种测定方法用于球体实验，以研究细胞特性和对治疗的反应。这些包括肿瘤生物学中用于确定肿瘤净反应的许多技术，如体积变化、组织学和净细胞存活，以及涉及细胞亚群分离的更直接的测定方法。还开发了方法来研究电离辐射或其他细胞毒性剂后的原位修复和再增殖过程。
