来自圆山药块茎的转化酶抑制剂的纯化与特性分析

Purification and characterization of invertase inhibitors from Dioscorea rotundata tuber.

作者信息

Nok A J, Igwe U A, Pai S J

机构信息

Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.

出版信息

J Enzyme Inhib. 1992;6(2):175-80. doi: 10.3109/14756369209040749.

DOI:10.3109/14756369209040749

PMID:1284434

Abstract

Three invertase inhibitors (A), (B) and (C) from Dioscorea rotundata tuber were resolved on DEAE-cellulose ion exchanger. Two of the inhibitors, (B) and (C), were proteins and homogenous on polyacrylamide gels Mr 21,000 +/- 85 and 26,982 +/- 40/36,307 +/- 50 respectively. The inhibitors (B) and (C) were inactivated at 60 degrees C and had activity-pH optima at 5.2 and 6.4 respectively. (B) and (C) were non competitive inhibitors of invertase from yam and other sources.

摘要

从圆山药块茎中分离出三种转化酶抑制剂（A）、（B）和（C），它们在DEAE - 纤维素离子交换剂上得以分离。其中两种抑制剂（B）和（C）是蛋白质，在聚丙烯酰胺凝胶上呈现均一性，其分子量分别为21,000 ± 85和26,982 ± 40/36,307 ± 50。抑制剂（B）和（C）在60℃时失活，活性pH最适值分别为5.2和6.4。（B）和（C）是山药及其他来源转化酶的非竞争性抑制剂。