Ozkan Keramettin Uğur, Kücükaydin Mustafa, Muhtaroğlu Sabahattin, Kontaş Olgun, Karaca Fahri
Department of Pediatric Surgery, KSU Medical School, Kahramanmaras, Turkey.
Urol Int. 2003;71(1):73-6. doi: 10.1159/000071098.
The aim of this study was to evaluate contralateral testicular damage (CTD) following unilateral blunt testicular trauma (BTT) and testicular capsule laceration (TCL) by the serum inhibin B level which is an accepted marker of spermatogenesis.
Fifty peripubertal male Wistar albino rats were divided into 5 groups each containing 10 rats. Group 1 was the control group. Group 2 was the BTT group in which the right testicle was placed on a firm surface and a metal rod weighing 215 g was dropped onto the testicle from a height of 5.5 cm. Group 3 was the TCL group in which right testicular tunica albuginea was lacerated using the needle of 4/0 silk suture. Group 4 had right orchiectomy initially. Group 5 was the sham group. In all groups, 3-ml blood samples were taken and bilateral orchiectomies were performed 6 weeks after initial manipulations.
Groups 2 and 3 had decreased inhibin B levels (p < 0.001), although the orchiectomy group had normal levels. Histological analyses showed lower Johnsen scores for both trauma groups in the ipsilateral and contralateral testes (p < 0.05).
Serum inhibin B levels decrease following unilateral testicular trauma reflecting CTD.
本研究旨在通过血清抑制素B水平评估单侧钝性睾丸损伤（BTT）和睾丸白膜撕裂伤（TCL）后对侧睾丸损伤（CTD），血清抑制素B是公认的精子发生标志物。
50只青春期前雄性Wistar白化大鼠分为5组，每组10只。第1组为对照组。第2组为BTT组，将右侧睾丸置于坚硬表面，从5.5厘米高度将一根重215克的金属棒掉落至睾丸上。第3组为TCL组，使用4/0丝线缝针划破右侧睾丸白膜。第4组最初进行右侧睾丸切除术。第5组为假手术组。所有组均采集3毫升血样，并在初次操作6周后进行双侧睾丸切除术。
第2组和第3组抑制素B水平降低（p < 0.001），而睾丸切除组水平正常。组织学分析显示，两个创伤组同侧和对侧睾丸的约翰森评分均较低（p < 0.05）。
单侧睾丸损伤后血清抑制素B水平降低，反映了对侧睾丸损伤。
