Todorić Davor, Durdov Merica Glavina, Tandara Marijan, Čapkun Vesna, Jurić Ivo, Biočić Mihovil, Meštrović Jakov, Pogorelić Zenon
Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000 Split, Croatia.
J Pediatr Urol. 2014 Dec;10(6):1063-9. doi: 10.1016/j.jpurol.2014.03.012. Epub 2014 May 2.
Open testicular biopsy (OTB) is one of the options to accurately assess fertility potential of the undescended testis. The aim of the study was to investigate consequences of OTB in prepubertal rats on their adulthood fertility.
Thirty-eight prepubertal male rats were divided into three groups depending on day 20 procedure. The first group was the control group, the second sham operated and the third has left OTB. Bilateral orchiectomy was performed on day 70 to all groups, with determination of serum inhibin B and follicle stimulating hormone (FSH). Removed testes were compared according to the weight, volume, spermatogenesis, histological and apoptotic changes in both testes with differences in serum levels of inhibin B and FSH.
Ipsilateral testicular weight, volume, and spermatogenesis reduction with a reduction of tubular number, diameter and germinative epithelium was found in OTB group. Significant increase in apoptotic index was found in biopsied testis without compensatory hypertrophy of contralateral testis. Differences of inhibin B and FSH were not statistically significant among three groups.
OTB in prepubertal rats has detrimental effects on fertility in adulthood. It does not cause compensatory hypertrophy of the contralateral testis nor does it disturb serum levels of inhibin B and FSH.
开放性睾丸活检（OTB）是准确评估隐睾生育潜力的方法之一。本研究的目的是调查青春期前大鼠进行OTB对其成年后生育能力的影响。
根据第20天的操作，将38只青春期前雄性大鼠分为三组。第一组为对照组，第二组为假手术组，第三组进行左侧OTB。在第70天对所有组进行双侧睾丸切除术，测定血清抑制素B和卵泡刺激素（FSH）。根据睾丸重量、体积、精子发生、组织学和凋亡变化以及血清抑制素B和FSH水平的差异，对切除的睾丸进行比较。
OTB组同侧睾丸重量、体积和精子发生减少，曲细精管数量、直径和生殖上皮减少。活检睾丸的凋亡指数显著增加，对侧睾丸无代偿性肥大。三组间抑制素B和FSH的差异无统计学意义。
青春期前大鼠进行OTB对成年后的生育能力有不利影响。它不会导致对侧睾丸的代偿性肥大，也不会干扰血清抑制素B和FSH水平。
