Pari L, Venkateswaran S
Department of Biochemistry, Faculty of Science, Annamalai University, Tamil Nadu, India.
Braz J Med Biol Res. 2003 Jul;36(7):861-70. doi: 10.1590/s0100-879x2003000700006. Epub 2003 Jun 26.
Changes in the structural and functional properties of collagen caused by advanced glycation might be of importance for the etiology of late complications in diabetes. The present study was undertaken to investigate the influence of oral administration of aqueous pod extract (200 mg/kg body weight) of Phaseolus vulgaris, an indigenous plant used in Ayurvedic Medicine in India, on collagen content and characteristics in the tail tendon of streptozotocin-diabetic rats. In diabetic rats, collagen content (117.01 6.84 mg/100 mg tissue) as well as its degree of cross-linking was increased, as shown by increased extent of glycation (21.70 0.90 g glucose/mg collagen), collagen-linked fluorescence (52.8 3.0 AU/ mol hydroxyproline), shrinkage temperature (71.50 2.50 C) and decreased acid (1.878 0.062 mg hydroxyproline/100 mg tissue) and pepsin solubility (1.77 0.080 mg hydroxyproline/100 mg tissue). The alpha/ ratio of acid- (1.69) and pepsin-soluble (2.00) collagen was significantly decreased in streptozotocin-diabetic rats. Administration of P. vulgaris for 45 days to streptozotocin-diabetic rats significantly reduced the accumulation and cross-linking of collagen. The effect of P. vulgaris was compared with that of glibenclamide, a reference drug administered to streptozotocin-diabetic rats at the dose of 600 g/kg body weight for 45 days by gavage. The effects of P. vulgaris (collagen content, 64.18 1.97; extent of glycation, 12.00 0.53; collagen-linked fluorescence, 33.6 1.9; shrinkage temperature, 57.0 1.0; extent of cross-linking - acid-soluble collagen, 2.572 0.080, and pepsin-soluble collagen, 2.28 0.112) were comparable with those of glibenclamide (collagen content, 71.5 2.04; extent of glycation, 13.00 0.60; collagen-linked fluorescence, 38.9 2.0; shrinkage temperature, 59.0 1.5; extent of cross-linking - acid-soluble collagen, 2.463 0.078, and pepsin-soluble collagen, 2.17 0.104). In conclusion, administration of P. vulgaris pods had a positive influence on the content of collagen and its properties in streptozotocin-diabetic rats.
晚期糖基化引起的胶原蛋白结构和功能特性的变化可能对糖尿病晚期并发症的病因学具有重要意义。本研究旨在调查口服印度阿育吠陀医学中使用的本土植物菜豆的水提物（200毫克/千克体重）对链脲佐菌素诱导的糖尿病大鼠尾腱中胶原蛋白含量和特性的影响。在糖尿病大鼠中，胶原蛋白含量（117.01±6.84毫克/100毫克组织）及其交联程度增加，表现为糖基化程度增加（21.70±0.90微克葡萄糖/毫克胶原蛋白）、胶原连接荧光（52.8±3.0任意单位/摩尔羟脯氨酸）、收缩温度（71.50±2.50℃）以及酸性（1.878±0.062毫克羟脯氨酸/100毫克组织）和胃蛋白酶溶解度降低（1.77±0.080毫克羟脯氨酸/100毫克组织）。链脲佐菌素诱导糖尿病大鼠中酸性（1.69）和胃蛋白酶可溶性（2.00）胶原蛋白的α/β比值显著降低。给链脲佐菌素诱导的糖尿病大鼠连续45天给予菜豆可显著降低胶原蛋白的积累和交联。将菜豆的效果与格列本脲进行比较，格列本脲是一种给链脲佐菌素诱导的糖尿病大鼠按600微克/千克体重的剂量灌胃45天的参考药物。菜豆的效果（胶原蛋白含量，64.18±1.97；糖基化程度，12.00±0.53；胶原连接荧光，33.6±1.9；收缩温度，57.0±1.0；交联程度 - 酸性可溶性胶原蛋白，2.572±0.080，胃蛋白酶可溶性胶原蛋白，2.28±0.112）与格列本脲的效果（胶原蛋白含量，71.5±2.04；糖基化程度，13.00±0.60；胶原连接荧光，38.9±2.0；收缩温度，59.0±1.5；交联程度 - 酸性可溶性胶原蛋白，2.463±0.078，胃蛋白酶可溶性胶原蛋白，2.17±0.104）相当。总之，给链脲佐菌素诱导的糖尿病大鼠给予菜豆荚对胶原蛋白的含量及其特性具有积极影响。
