Ramesh Balakrishnan, Pugalendi Kodukkur Viswanathan
Department of Biochemistry, Faculty of Science, Annamalai University, Tamilnadu, India.
Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):73-7. doi: 10.1111/j.1742-7843.2007.00090.x.
Diabetes mellitus is known to affect collagen in various tissues. Umbelliferone (7-hydroxycoumarin), a natural antioxidant and benzopyrone, is found in golden apple (Aegle marmelos Correa) and bitter orange (Citrus aurantium). Plant-derived phenolic coumarins have been shown to act as dietary antioxidants. In this study, we have investigated the influence of umbelliferone on collagen content and its effects on the tail tendon in streptozotocin-diabetic rats. Male albino Wistar rats (180-200 g) were made diabetic by intraperitoneal administration of streptozotocin (40 mg/kg). Normal and diabetic rats were treated with umbelliferone for 45 days. Diabetic rats had increased glucose and decreased insulin levels. Tail tendons of diabetic rats had increased total collagen, glycation and fluorescence, and decreased levels of neutral, acid and pepsin-soluble collagens. We have studied the effect of umbelliferone on haemostatic function because umbelliferone is also a coumarin derivative like the anticoagulant, warfarin. Diabetic rats had a significant decrease in prothrombin, clotting and bleeding time, and treatment with umbelliferone made these parameters almost normal. Our results show that umbelliferone controls glycaemia and has a beneficial effect on collagen content and its properties, i.e. collagen related parameters, in the tail tendon, which indicates recovery from the risk (recovery of animals from the risk of complications) of collagen-mediated diabetic polyneuropathy and diabetic nephropathy.
众所周知，糖尿病会影响各种组织中的胶原蛋白。伞形花内酯（7-羟基香豆素）是一种天然抗氧化剂和苯并吡喃酮，存在于金苹果（木苹果柯里亚）和苦橙（酸橙）中。植物来源的酚类香豆素已被证明可作为膳食抗氧化剂。在本研究中，我们研究了伞形花内酯对链脲佐菌素诱导的糖尿病大鼠胶原蛋白含量的影响及其对尾腱的作用。雄性白化Wistar大鼠（180 - 200克）通过腹腔注射链脲佐菌素（40毫克/千克）诱导糖尿病。正常和糖尿病大鼠用伞形花内酯治疗45天。糖尿病大鼠血糖升高，胰岛素水平降低。糖尿病大鼠的尾腱总胶原蛋白、糖基化和荧光增加，中性、酸性和胃蛋白酶可溶性胶原蛋白水平降低。我们研究了伞形花内酯对止血功能的影响，因为伞形花内酯也是一种香豆素衍生物，类似于抗凝剂华法林。糖尿病大鼠的凝血酶原、凝血和出血时间显著缩短，用伞形花内酯治疗后这些参数几乎恢复正常。我们的结果表明，伞形花内酯可控制血糖，并对尾腱中的胶原蛋白含量及其特性（即与胶原蛋白相关的参数）具有有益作用，这表明从胶原蛋白介导的糖尿病多发性神经病和糖尿病肾病的风险中恢复（动物从并发症风险中恢复）。
