Kretschmer V, Fauth F, Heins M, Dietrich G, Schulzki T, Bachem G, Brand O
Abteilung Transfusionsmedizin und Gerinnungsphysiologie, Universitätskliniken, Marburg, BRD.
Beitr Infusionsther. 1992;30:166-9.
We report on an alternative to filtration for the preparation of leukocyte-poor red cell concentrates (LP-RCC). It is based on the method of Schneider. Using RCC with buffy coat it is comparably effective in leukocyte reduction [98.3 +/- 1.0%, (3.7 +/- 2.6) x 10(6) leukocytes] and more effective in platelet reduction (96.9 +/- 2.5%). Addition of PAGGS-M before heating (30 min, 37 degrees C) as well as after preparation significantly reduces hemolysis (free hemoglobin, LDH, HBDH) and improves the quality of the LP-RCC (ATP, 2,3-DPG) during storage for 24 h after preparation. LP-RCCs prepared with PAGGS-M after 6-day storage show still better quality than before preparation and about the same quality as LP-RCCs 24 h after conventional preparation with saline solution. In conclusion, by use of PAGGS-M and sterile docking LP-RCCs of adequate quality for 6-day storage can be prepared, improving the supply of the patients concerned.
我们报告了一种用于制备少白细胞红细胞浓缩物（LP - RCC）的替代过滤方法。它基于施奈德的方法。使用带有血沉棕黄层的RCC进行白细胞去除时效果相当（白细胞减少率为98.3±1.0%，白细胞数为(3.7±2.6)×10⁶），而血小板减少效果更显著（96.9±2.5%）。在加热前（30分钟，37℃）以及制备后添加PAGGS - M可显著降低溶血（游离血红蛋白、乳酸脱氢酶、羟丁酸脱氢酶），并在制备后24小时的储存期间改善LP - RCC的质量（三磷酸腺苷、2,3 - 二磷酸甘油酸）。用PAGGS - M制备的LP - RCC在储存6天后显示出仍比制备前质量更好，且与用盐溶液常规制备24小时后的LP - RCC质量大致相同。总之，通过使用PAGGS - M和无菌对接，可以制备出质量合格的用于6天储存的LP - RCC，改善相关患者的供应情况。
