Giannitsis D J, Hofstätter I, Wild J, Häcker-Shahin B
Klinikum Karlsruhe, Blutspendezentrale, BRD.
Beitr Infusionsther. 1992;30:436-8.
A rapid manual test for the detection of red cell antibodies called SLP assay has been developed and compared with the sensitivity of the antiglobulin assay. Acid-soluble proteins (SLP) from human leukocytes cause aggregation of human red blood cells. SLP represents a group of proteins consisting of 5 fractions of different positively charged macromolecules, which are able to reduce the negative charge of the red cells. Reduction of the negative charge results in a nonspecific hemagglutination of different strengths, depending on the SLP fractions used. This hemagglutination can be reversed by neutralizing the SLP with heparin. In the case of blood group-antibody-mediated aggregation the hemagglutination is nonreversible despite neutralization with heparin and remains stable for several hours. Because of the high sensitivity of the SLP assay all blood group antibodies from the IgM type as well as from the IgG type are detectable even in low concentrations. The sensitivity of the SLP assay is comparable to the antiglobulin assay.
一种名为SLP检测法的用于检测红细胞抗体的快速手工检测方法已被开发出来，并与抗球蛋白检测法的灵敏度进行了比较。来自人类白细胞的酸溶性蛋白（SLP）会导致人类红细胞聚集。SLP代表一组由5种不同带正电荷的大分子组分组成的蛋白质，它们能够减少红细胞的负电荷。负电荷的减少会导致不同强度的非特异性血凝，具体强度取决于所使用的SLP组分。这种血凝可以通过用肝素中和SLP来逆转。在血型抗体介导的聚集情况下，尽管用肝素中和，血凝仍不可逆，并能保持数小时稳定。由于SLP检测法具有高灵敏度，即使是低浓度的IgM型和IgG型血型抗体都能被检测到。SLP检测法的灵敏度与抗球蛋白检测法相当。
