Hugo T, Wüst T, Beeser H P
University Department of Transfusion Medicine, Freiburg, FRG.
Beitr Infusionsther. 1992;30:49-52.
Stored serum samples from 7,179 nonselected blood donors were tested for anti-HCV using Ortho EIA first generation. Results were compared to data acquired by anti-HBc testing and ALT levels found in routine testing. 24 donors (0.33%) were repeatedly reactive with Ortho HCV EIA, 230 (3.20%) were anti-HBc-positive and 138 (1.92%) had raised ALT levels > or = 36 IU/l. A low correlation was found between HCV antibody screening with EIA and surrogate testing. When tested in addition with the Abbott HCV EIA, 20 of the 24 Ortho EIA-positive subjects showed a positive reaction. In the Abbott neutralization test 13 of these 20 (65%) were reactive. 8 (33.33%) of the 24 Ortho-EIA-positive donors were positive in the two-antigen-RIBA (first generation), 8 were indeterminate and 8 were nonreactive. The neutralization test and the RIBA can be used as supplementary tests fo further analyze HCV-EIA-positive specimens.
使用第一代Ortho酶免疫测定法对7179名非选择性献血者的储存血清样本进行抗丙型肝炎病毒（anti-HCV）检测。将结果与通过抗乙肝核心抗体（anti-HBc）检测获得的数据以及常规检测中发现的丙氨酸氨基转移酶（ALT）水平进行比较。24名献血者（0.33%）经Ortho HCV酶免疫测定法反复呈阳性反应，230名（3.20%）抗-HBc呈阳性，138名（1.92%）的ALT水平升高至≥36 IU/L。酶免疫测定法进行的丙型肝炎病毒抗体筛查与替代检测之间存在低相关性。当另外用雅培丙型肝炎病毒酶免疫测定法检测时，24名Ortho酶免疫测定法阳性受试者中有20名呈阳性反应。在雅培中和试验中，这20名受试者中有13名（65%）呈阳性反应。24名Ortho酶免疫测定法阳性献血者中有8名（33.33%）在双抗原重组免疫印迹法（第一代）中呈阳性，8名结果不确定，8名无反应。中和试验和重组免疫印迹法可作为补充检测，用于进一步分析酶免疫测定法检测丙型肝炎病毒阳性的标本。
