Zimmermann R, König V, Bauditz J, Zeiler T, Zingsem J, Heuft H G, Hopf U, Huhn D, Eckstein R
Abteilung Innere Medizin, Universitätsklinikum Rudolf-Virchow, Freie Universität Berlin, BRD.
Beitr Infusionsther. 1992;30:38-41.
Hepatitis C virus (HCV) is responsible for the majority of cases of transfusion-related hepatitis. We performed a first-generation anti-HCV EIA in 665 repeat and 168 first-time blood donors from Berlin. 4.7 and 4.2%, respectively, showed at least one indeterminate or positive result. We further looked for HCV genome in the plasma of 20 donors with reactive anti-HCV-EIA doing a polymerase chain reaction (nested PCR). The control group consisted of 20 patients with chronic hepatitis C. The PCR was negative in all examined blood donors, but was positive in 17 of 20 controls. These findings raise the question, if a positive anti-HCV test correlates with infectiosity.
丙型肝炎病毒（HCV）是大多数输血相关肝炎病例的病因。我们对来自柏林的665名重复献血者和168名首次献血者进行了第一代抗-HCV酶免疫测定（EIA）。分别有4.7%和4.2%的人显示至少有一个不确定或阳性结果。我们进一步通过聚合酶链反应（巢式PCR）在20名抗-HCV-EIA反应性献血者的血浆中寻找HCV基因组。对照组由20名慢性丙型肝炎患者组成。所有接受检测的献血者PCR结果均为阴性，但20名对照组中有17名呈阳性。这些发现提出了一个问题，即抗-HCV检测呈阳性是否与传染性相关。
