Csaba G, Hegyesi H, Török O
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Acta Physiol Hung. 1992;79(2):213-7.
When the cells of the Chang cell line came into interaction with a hormone (insulin) an imprinting-like phenomenon took place. The binding capacity of the receptors strengthened and this feature was transmitted to the descendant generations. The quality of the nutrient medium influenced the development of imprinting, when the cells were maintained in a medium containing 2% serum it was more difficult to evoke imprinting than in case the cells were kept in a medium containing 10% serum. If the cells were cultured kept in Tyrode (physiological) solution for 24 hours the possibility to evoke imprinting was lost. Difference could be observed between the behaviour of receptors in nuclear membrane and that of receptors in the plasma membrane; i.e. changes were more dynamic in the plasma membrane.
当Chang细胞系的细胞与一种激素（胰岛素）相互作用时，发生了一种类似印记的现象。受体的结合能力增强，且这一特性传递给了后代细胞。营养培养基的质量影响印记的发展，当细胞培养于含2%血清的培养基中时，比培养于含10%血清的培养基中更难诱发印记。如果将细胞在Tyrode（生理）溶液中培养24小时，则失去了诱发印记的可能性。核膜受体和质膜受体的行为存在差异，即质膜中的变化更具动态性。
