Hegyesi H, Csaba G
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Cytobios. 1992;72(290-291):191-6.
About 4 h following treatment with 10(-6) - 10(-10) M insulin (imprinting) there was a down-regulation both in the plasma membrane and in the nuclear envelope of Tetrahymena, i.e. there was a decrease in the binding value related to the control. In each concentration tested the imprinting resulted in increased FITC-insulin binding after 24 and 48 h of treatment; the imprinting was independent of the concentrations. In the case of the plasma membrane the power of binding was directly proportional to the increase of the hormone concentration participating in the imprinting. For the nuclear envelope the optimal concentration was 10(-8) M. At very low concentrations (10(-10) M) the binding resulting in imprinting was insignificant in the plasma membrane but it was still present in the nucleus.
用10⁻⁶ - 10⁻¹⁰ M胰岛素处理（印记）约4小时后，四膜虫的质膜和核膜均出现下调，即与对照相比结合值降低。在每个测试浓度下，印记处理在处理24小时和48小时后均导致异硫氰酸荧光素 - 胰岛素结合增加；印记与浓度无关。就质膜而言，结合能力与参与印记的激素浓度增加成正比。对于核膜，最佳浓度为10⁻⁸ M。在非常低的浓度（10⁻¹⁰ M）下，导致印记的结合在质膜中不显著，但在细胞核中仍然存在。
