Lähdeaho M L, Mäki M, Parkkonen P, Kantonen T, Lehtinen M
Institute of Biomedical Sciences, University of Tampere, Finland.
Acta Virol. 1992 Dec;36(6):524-32.
We evaluated possibilities to analyze serum antibodies to non-structural (peptides derived from adenovirus E1b protein) and structural (hexon) adenovirus antigens by ELISA. Synthetic dodecapeptides covering a putative A-gliadin cross-reactive antigenic determinant of the E1b protein were used. The aminoterminus of the peptides appeared to be important for antibody binding but the exact sequence of a possible common B-cell epitope within the peptides remained open. Coupling of the peptides to a carrier protein was essential for ELISA analyses of serum antipeptide antibodies. IgA antibodies to both adenovirus derived E1b peptides and hexon antigen could be detected already two weeks after the onset of an acute adenovirus infection, while antipeptide IgG antibodies were seen in a restricted number of patients only.
我们评估了通过酶联免疫吸附测定（ELISA）分析血清中针对非结构（源自腺病毒E1b蛋白的肽段）和结构（六邻体）腺病毒抗原的抗体的可能性。使用了覆盖E1b蛋白假定的A-麦醇溶蛋白交叉反应性抗原决定簇的合成十二肽。肽段的氨基末端似乎对抗体结合很重要，但肽段内可能的共同B细胞表位的确切序列仍未明确。肽段与载体蛋白的偶联对于血清抗肽抗体的ELISA分析至关重要。在急性腺病毒感染开始后两周就可以检测到针对腺病毒衍生的E1b肽段和六邻体抗原的IgA抗体，而仅在少数患者中观察到抗肽IgG抗体。
