Zhang M J, Wang M X, Jiang S Z, Xiu Z Z, Ma W Y
Department of Microbiology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Acta Virol. 1992 Dec;36(6):533-40.
Six mouse monoclonal antibodies (MoAbs) against Japanese encephalitis virus (JEV) were prepared and analyzed with indirect immunofluorescence assay (IFA), enzyme linked immunosorbent assay (ELISA), haemagglutination inhibition test (HI), neutralization test (NT), antibody dependent cell mediated cytotoxicity (ADCC) assay, antigenic site specific analysis and relative affinity measurement. These MoAbs could be divided into three classes by indirect immunofluorescence cross reactivity among four flaviviruses, 2H4, 2F2, and nG2 were type specific; 2D2 and mC3 were subgroup specific; and mG9 was family specific. 2H4 and 2F2 had higher neutralization activity, 2D2 and mC3 had the function of inducing ADCC effect, mG9 had higher titer in HI. The six MoAbs recognized five antigenic sites on JEV envelope glycoprotein, 2H4 and 2F2 recognized the same or very similar antigenic site and their relative affinity was ranked as: nG2 > 2H4 > 2D2 > mG9 > 2F2 > mC3.
制备了6株抗日本脑炎病毒（JEV）的小鼠单克隆抗体（MoAbs），并通过间接免疫荧光试验（IFA）、酶联免疫吸附试验（ELISA）、血凝抑制试验（HI）、中和试验（NT）、抗体依赖性细胞介导的细胞毒性（ADCC）试验、抗原位点特异性分析和相对亲和力测定进行分析。根据4种黄病毒之间的间接免疫荧光交叉反应性，这些单克隆抗体可分为3类，2H4、2F2和nG2具有型特异性；2D2和mC3具有亚组特异性；mG9具有属特异性。2H4和2F2具有较高的中和活性，2D2和mC3具有诱导ADCC效应的功能，mG9在HI中具有较高的效价。这6株单克隆抗体识别JEV包膜糖蛋白上的5个抗原位点，2H4和2F2识别相同或非常相似的抗原位点，它们的相对亲和力排序为：nG2 > 2H4 > 2D2 > mG9 > 2F2 > mC3。
