Gupta A K, Bhattacharya S, Lad V J, Sarthi S A, Kutubuddin M, Ghosh S N, Banerjee K
National Institute of Virology, Pune, India.
Acta Virol. 1992 Aug;36(4):401-11.
An immunoglobulin G (IgG2b) class of monoclonal antibody (MoAb, NHA-1) raised against Japanese encephalitis virus (JEV) E glycoprotein, reacted with the viral antigen expressed in cytoplasm of the infected cells and also with the cell nuclei, by an indirect fluorescent antibody technique (FA). The NHA-1 reactivity to nuclei was found to be due to its recognizing a JEV cross-reactive epitope present on the nuclear histones. Adsorption with calf thymus histones (type II-AS) showed a drop in NHA-1 reactivity to both JEV and histones by an enzyme-linked immunosorbent assay (ELISA) and indirect FA; the drop was higher against the histones. The MoAb recognized specifically the viral antigens expressed on the infected porcine kidney cell surface by a modified indirect FA. ELISA carried out with glutaraldehyde-fixed antigens showed an almost 2-fold increase in the reactivity over unfixed JEV antigen but none for the histones. Thus, the results indicate that histones share a sequential homology with E glycoprotein of JEV, which might lead to an autoimmune disorder induced due to the molecular mimicry between these two antigens.
一种针对日本脑炎病毒（JEV）E糖蛋白产生的免疫球蛋白G（IgG2b）类单克隆抗体（MoAb，NHA-1），通过间接荧光抗体技术（FA）与感染细胞胞质中表达的病毒抗原以及细胞核发生反应。发现NHA-1对细胞核的反应性是由于其识别核组蛋白上存在的JEV交叉反应表位。用小牛胸腺组蛋白（II-AS型）吸附后，通过酶联免疫吸附测定（ELISA）和间接FA发现NHA-1对JEV和组蛋白的反应性均下降；对组蛋白的下降幅度更大。通过改良的间接FA，该单克隆抗体特异性识别感染的猪肾细胞表面表达的病毒抗原。用戊二醛固定抗原进行的ELISA显示，与未固定的JEV抗原相比，反应性几乎增加了2倍，但对组蛋白无反应。因此，结果表明组蛋白与JEV的E糖蛋白具有序列同源性，这可能导致由于这两种抗原之间的分子模拟而引发自身免疫性疾病。
