Brown Gregory G, Formanová Natasa, Jin Hua, Wargachuk Richard, Dendy Charles, Patil Prashant, Laforest Martin, Zhang Jinfa, Cheung Wing Y, Landry Benoit S
Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1.
Plant J. 2003 Jul;35(2):262-72. doi: 10.1046/j.1365-313x.2003.01799.x.
A single radish nuclear gene, Rfo, restores Ogura (ogu) cytoplasmic male sterility (CMS) in Brassica napus. A map-based cloning approach relying on synteny between radish and Arabidopsis was used to clone Rfo. A radish gene encoding a 687-amino-acid protein with a predicted mitochondrial targeting pre-sequence was found to confer male fertility upon transformation into ogu CMS B. napus. This gene, like the recently described Petunia Rf gene, codes for a pentatricopeptide repeat (PPR)-containing protein with multiple, in this case 16, PPR domains. Two similar genes that do not appear to function as Rfo flank this gene. Comparison of the Rfo region with the syntenic Arabidopsis region indicates that a PPR gene is not present at the Rfo-equivalent site in Arabidopsis, although a smaller and related PPR gene is found about 40 kb from this site. The implications of these findings for the evolution of restorer genes and other PPR encoding genes are discussed.
一个单一的萝卜核基因Rfo可恢复甘蓝型油菜中Ogura（ogu）细胞质雄性不育（CMS）。采用基于图谱克隆的方法，依靠萝卜与拟南芥之间的共线性来克隆Rfo。发现一个编码含687个氨基酸且带有预测的线粒体靶向前序列的蛋白质的萝卜基因，在转化到ogu CMS甘蓝型油菜中时可赋予雄性育性。该基因与最近描述的矮牵牛Rf基因一样，编码一种含有五肽重复序列（PPR）的蛋白质，在此情况下有多个（16个）PPR结构域。该基因两侧有两个似乎不具有Rfo功能的相似基因。将Rfo区域与拟南芥的共线区域进行比较表明，在拟南芥中与Rfo等效的位点不存在PPR基因，不过在距该位点约40 kb处发现了一个较小的相关PPR基因。讨论了这些发现对恢复基因和其他PPR编码基因进化的影响。
