Wang Xuesong, Liang Xingyu, Wang Rui, Gao Yuan, Li Yun, Shi Haoran, Gong Wanzhuo, Saleem Saira, Zou Qiong, Tao Lanrong, Kang Zeming, Yang Jin, Yu Qin, Wu Qiaobo, Liu Hailan, Fu Shaohong
Maize Research Institute of Sichuan Agricultural University, Chengdu, China.
National Rapeseed Genetic Improvement Center, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu Research Branch, Chengdu, China.
Front Genet. 2025 Jan 6;15:1521277. doi: 10.3389/fgene.2024.1521277. eCollection 2024.
The Ogura cytoplasmic male sterility (CMS) line of has gained significant attention for its use in harnessing heterosis. It remains unaffected by temperature and environment and is thorough and stable. The Ogura cytoplasmic restorer line of is derived from the distant hybridization of and , but it carried a large number of radish fragments into , because there is no homologous allele of the restorer gene in , transferring it becomes challenging. In this study, the double haploid induction line in was used as the male parent for hybridization with the Ogura CMS of Surprisingly, fertile plants appeared in the offspring. Further analysis revealed that the cytoplasmic type, ploidy, and chromosome number of the fertile offspring were consistent with the sterile female parent. Moreover, the mitochondrial genome similarity between the fertile offspring and the sterile female parent was 97.7% indicates that the cytoplasm of the two is the same, while the nuclear gene difference between fertile offspring and sterile female parent was only 10.33%, indicates that new genes appeared in the offspring. To further investigate and locate the restorer gene, the BSA method was employed to construct extreme mixed pools. As a result, the restorer gene was mapped to three positions: A09 chromosome 10.99-17.20 Mb, C03 chromosome 5.07-5.34 Mb, and C09 chromosome 18.78-36.60 Mb. The experimental results have proved that induction does produce restorer genes. The induction of the Ogura CMS restorer gene through DH induction line provides a promising new approach for harnessing heterosis in .
Ogura细胞质雄性不育（CMS）系因其在杂种优势利用中的应用而备受关注。它不受温度和环境影响，彻底且稳定。Ogura细胞质恢复系由[具体物种1]和[具体物种2]的远缘杂交而来，但它将大量萝卜片段带入了[目标物种]，因为[目标物种]中没有恢复基因的同源等位基因，转移它具有挑战性。在本研究中，[目标物种]中的双单倍体诱导系被用作父本与[目标物种]的Ogura CMS杂交。令人惊讶的是，后代中出现了可育植株。进一步分析表明，可育后代的细胞质类型、倍性和染色体数与不育母本一致。此外，可育后代与不育母本之间的线粒体基因组相似度为97.7%，表明两者细胞质相同，而可育后代与不育母本之间的核基因差异仅为10.33%，表明后代中出现了新基因。为了进一步研究和定位恢复基因，采用了BSA法构建极端混合池。结果，恢复基因被定位到三个位置：A09染色体10.99 - 17.20 Mb、C03染色体5.07 - 5.34 Mb和C09染色体18.78 - 36.60 Mb。实验结果证明诱导确实产生了恢复基因。通过DH诱导系诱导Ogura CMS恢复基因，为[目标物种]杂种优势的利用提供了一种有前景的新方法。
