Li Bo-qing, Zhang Jian-zhong, Zou Qing-hua, He Li-hua, Yan Xiao-mei
Department of Diagnosis, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jun;24(6):439-42.
To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.
The whole-cell proteins of H. pylori were separated by two-dimensional electrophoresis (2-DE). The protein maps of four H. pylori strains associated with gastric carcinoma and nine strains that isolated from patients with non-gastric carcinoma were then compared by ImageMaster 2D v3.1. MALDI-TOF mass spectrometry was performed to identify the proteins of interest. The proteins were searched by software mascot and identified by peptide fingerprint map.
Three proteins seemed to be associated with gastric carcinoma including acylneuraminate cytidylyltransferase with Mowse score 79 with the sequence coverage of 32%. The other two had no unambiguous protein to match.
Acylneuraminate cytidylyltransferase seemed to be a specific H. pylori protein associated with the presence of gastric carcinoma. Other two were novel proteins that might be associated with gastric carcinoma. However, the mechanism needs to be explored.
鉴定与胃癌相关的幽门螺杆菌（H. pylori）特异性蛋白。
采用双向电泳（2-DE）分离幽门螺杆菌全细胞蛋白。然后用ImageMaster 2D v3.1软件比较4株与胃癌相关的幽门螺杆菌菌株和9株从非胃癌患者中分离出的菌株的蛋白质图谱。进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）分析以鉴定感兴趣的蛋白质。用软件mascot搜索蛋白质并用肽指纹图谱进行鉴定。
三种蛋白质似乎与胃癌相关，其中包括唾液酸胞苷酰转移酶，其Mowse评分为79，序列覆盖率为32%。另外两种蛋白质没有明确匹配的蛋白。
唾液酸胞苷酰转移酶似乎是一种与胃癌存在相关的幽门螺杆菌特异性蛋白。另外两种是可能与胃癌相关的新蛋白。然而，其机制有待探索。
