[与胃癌相关的幽门螺杆菌菌株蛋白质图谱的初步分析]

[Primary analysis on protein maps of Helicobacter pylori strains associated with gastric carcinoma].

作者信息

Li Bo-qing, Zhang Jian-zhong, Zou Qing-hua, He Li-hua, Yan Xiao-mei

机构信息

Department of Diagnosis, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jun;24(6):439-42.

PMID:12848904

Abstract

OBJECTIVE

To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.

METHODS

The whole-cell proteins of H. pylori were separated by two-dimensional electrophoresis (2-DE). The protein maps of four H. pylori strains associated with gastric carcinoma and nine strains that isolated from patients with non-gastric carcinoma were then compared by ImageMaster 2D v3.1. MALDI-TOF mass spectrometry was performed to identify the proteins of interest. The proteins were searched by software mascot and identified by peptide fingerprint map.

RESULTS

Three proteins seemed to be associated with gastric carcinoma including acylneuraminate cytidylyltransferase with Mowse score 79 with the sequence coverage of 32%. The other two had no unambiguous protein to match.

CONCLUSION

Acylneuraminate cytidylyltransferase seemed to be a specific H. pylori protein associated with the presence of gastric carcinoma. Other two were novel proteins that might be associated with gastric carcinoma. However, the mechanism needs to be explored.

摘要

目的

鉴定与胃癌相关的幽门螺杆菌（H. pylori）特异性蛋白。

方法

采用双向电泳（2-DE）分离幽门螺杆菌全细胞蛋白。然后用ImageMaster 2D v3.1软件比较4株与胃癌相关的幽门螺杆菌菌株和9株从非胃癌患者中分离出的菌株的蛋白质图谱。进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）分析以鉴定感兴趣的蛋白质。用软件mascot搜索蛋白质并用肽指纹图谱进行鉴定。