Hibasami Hiroshige, Yamada Yayoi, Moteki Hiroyuki, Katsuzaki Hirotaka, Imai Kunio, Yoshioka Kazumi, Komiya Takashi
Faculty of Medicine, Mie University, Tsu-city, Mie 514-0001, Japan.
Int J Mol Med. 2003 Aug;12(2):147-51.
Sesquiterpenes (costunolide and zaluzanin D) were purified from laurel (Laurus nobilis L.) and identified by Mass, and 1H- and 13C-NMR. These sesquiterpenes displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological changes of the nucleus, including chromatin condensation were induced in the HL-60 cells treated with the sesquiterpenes. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 11.4, 47.0, and 92.5% after a 3-day-treatment with 5, 10 and 15 micro M costunolide, respectively. The same analysis showed that the hypodiploid nuclei of HL 60 cells were increased to 12.4, 28.9 and 76.7% after a 3-day-treatment with 10, 15 and 20 micro M zaluzanin D, respectively. These findings suggest that growth inhibition by costunolide and zaluzanin D of HL-60 cells results from the induction of chromatin condensation in the HL-60 cells. On the other hand, fragmentations by these compounds of DNA to oligonucleosomal-sized fragments were not observed in the HL-60 cells.
倍半萜类化合物（木香烯内酯和扎鲁扎宁D）从月桂（月桂）中纯化出来，并通过质谱、1H-和13C-NMR进行鉴定。这些倍半萜类化合物对人早幼粒细胞白血病HL-60细胞显示出强烈的生长抑制作用。在用倍半萜类化合物处理的HL-60细胞中诱导了细胞核的凋亡形态变化，包括染色质浓缩。流式细胞术分析表明，用5、10和15微摩尔/升木香烯内酯处理3天后，HL-60细胞的亚二倍体核分别增加到11.4%、47.0%和92.5%。同样的分析表明，用10、15和20微摩尔/升扎鲁扎宁D处理3天后，HL-60细胞的亚二倍体核分别增加到12.4%、28.9%和76.7%。这些发现表明，木香烯内酯和扎鲁扎宁D对HL-60细胞的生长抑制是由HL-60细胞中染色质浓缩的诱导引起的。另一方面，在HL-60细胞中未观察到这些化合物将DNA切割成寡核小体大小片段的情况。
