Hibasami Hiroshige, Saitoh Kazumi, Katsuzaki Hirotaka, Imai Kunio, Aratanechemuge Yue, Komiya Takashi
Faculty of Medicine, Mie University, Mie 514-0001, Japan.
Int J Mol Med. 2004 Dec;14(6):1029-33.
2-O-methylisohemigossylic acid lactone, a sesquiterpene, was purified from roots of mokumen (Gossampinus malabarica) and identified by Mass, and (1)H- and (13)-NMR. This sesquiterpene displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological change of the nucleus, including chromatin condensation was induced in the HL-60 cells treated with the sesquiterpene. The fragmentation of DNA by the sesquiterpene to oligonucleosomal-sized fragments, a characteristic of apoptosis, was observed to be dose- and time-dependent in the HL-60 cells. Inhibitors of caspases suppressed the DNA fragmentation induced by the sesquiterpene. These findings suggest that growth inhibition by the sesquiterpene of HL-60 cells results from the induction of apoptosis by the sesqui-terpene, and that caspase cascade is involved in the induction of apoptosis by the compound in the HL-60 cells.
2-O-甲基异半棉酚内酯，一种倍半萜，从木棉（Gossampinus malabarica）的根中纯化得到，并通过质谱、氢核磁共振（¹H-NMR）和碳核磁共振（¹³C-NMR）进行鉴定。这种倍半萜对人早幼粒细胞白血病HL-60细胞显示出强烈的生长抑制作用。在用该倍半萜处理的HL-60细胞中诱导出细胞核的凋亡形态变化，包括染色质浓缩。在HL-60细胞中观察到该倍半萜将DNA切割成寡核小体大小的片段，这是凋亡的一个特征，且呈剂量和时间依赖性。半胱天冬酶抑制剂抑制了该倍半萜诱导的DNA片段化。这些发现表明，该倍半萜对HL-60细胞的生长抑制是由其诱导凋亡所致，且半胱天冬酶级联反应参与了该化合物在HL-60细胞中诱导凋亡的过程。
