Hibasami Hiroshige, Moteki Hiroyuki, Ishikawa Kengo, Katsuzaki Hirotaka, Imai Kunio, Yoshioka Kazumi, Ishii Yaeko, Komiya Takashi
Faculty of Medicine, Mie University, Tsu-city, Mie 514-0001, Japan.
Int J Mol Med. 2003 Jan;11(1):23-6.
Protodioscin (PD) was purified from fenugreek (Trigonella foenumgraecum L.) and identified by Mass, and 1H- and 13C-NMR. The effects of PD on cell viability in human leukemia HL-60 and human stomach cancer KATO III cells were investigated. PD displayed strong growth inhibitory effect against HL-60 cells, but weak growth inhibitory effect on KATO III cells. Morphological change showing apoptotic bodies was observed in the HL-60 cells treated with PD, but not in KATO III cells treated with PD. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 75.2, 96.3, and 100% after a 3-day treatment with 2.5, 5, and 10 microM PD, respectively. The fragmentation by PD of DNA to oligonucleosomal-sized fragments, that is a characteristic of apoptosis, was observed to be both concentration- and time-dependent in the HL-60 cells. These findings suggest that growth inhibition by PD of HL-60 cells results from the induction of apoptosis by this compound in HL-60 cells.
薯蓣皂苷元（PD）从胡芦巴（Trigonella foenumgraecum L.）中纯化得到，并通过质谱、1H-核磁共振和13C-核磁共振进行鉴定。研究了PD对人白血病HL-60细胞和人胃癌KATO III细胞活力的影响。PD对HL-60细胞显示出强烈的生长抑制作用，但对KATO III细胞的生长抑制作用较弱。在用PD处理的HL-60细胞中观察到显示凋亡小体的形态变化，但在用PD处理的KATO III细胞中未观察到。流式细胞术分析表明，在用2.5、5和10 microM PD处理3天后，HL-60细胞的亚二倍体核分别增加到75.2%、96.3%和100%。在HL-60细胞中观察到PD将DNA片段化为寡核小体大小的片段，这是凋亡的一个特征，且在HL-60细胞中呈浓度和时间依赖性。这些发现表明，PD对HL-60细胞的生长抑制是由该化合物在HL-60细胞中诱导凋亡所致。
