温度和宿主细胞类型对镰状肉孢子虫体外生长发育的影响。
Effects of temperature and host cell type on the in vitro growth and development of Sarcocystis falcatula.
作者信息
Elsheikha Hany M, Saeed Mahdi A, Fitzgerald Scott D, Murphy Alice J, Mansfield Linda S
机构信息
Molecular Epidemiology Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
出版信息
Parasitol Res. 2003 Sep;91(1):22-6. doi: 10.1007/s00436-003-0902-x. Epub 2003 Jul 8.
The growth and development of Sarcocystis falcatula in four different cultured cell lines [vero cells, bovine turbinate (BT) cells, equine dermal (ED) cells, and human Hep-2 cells] inoculated with culture-derived merozoites are described. Parasite yields, viability, and plaque forming efficiency were used to compare the growth between different cell lines. Additionally, each cell line was tested at two temperatures of incubation (35 degrees C and 37 degrees C). Based on yield, viability, and plaque forming efficiency, vero cells and BT cells supported growth of S. falcatula better than ED cells and Hep-2 cells. During an 18-day culture period, vero cells produced a mean total of 1.3x10(7) S. falcatula merozoites/T25 flask, BT cells 1.1x10(7), ED cells 0.9x10(7), and Hep-2 cells 0.7x10(7) merozoites/T25 flask. All experimental cell lines grew equally well at 35 degrees C and 37 degrees C. The type of host cells but not the temperature of incubation had a profound effect on the in vitro growth and proliferation of S. falcatula.
本文描述了用培养获得的裂殖子接种四种不同培养细胞系（非洲绿猴肾细胞、牛鼻甲细胞、马皮肤细胞和人喉表皮样癌细胞）后，猫肌囊尾蚴的生长和发育情况。用寄生虫产量、活力和空斑形成效率来比较不同细胞系间的生长情况。此外，每个细胞系在两个培养温度（35℃和37℃）下进行测试。基于产量、活力和空斑形成效率，非洲绿猴肾细胞和牛鼻甲细胞比马皮肤细胞和人喉表皮样癌细胞更有利于猫肌囊尾蚴的生长。在18天的培养期内，非洲绿猴肾细胞平均每个T25培养瓶产生1.3×10⁷个猫肌囊尾蚴裂殖子，牛鼻甲细胞产生1.1×10⁷个，马皮肤细胞产生0.9×10⁷个，人喉表皮样癌细胞产生0.7×10⁷个裂殖子/T25培养瓶。所有实验细胞系在35℃和37℃下生长情况相同。宿主细胞类型而非培养温度对猫肌囊尾蚴的体外生长和增殖有深远影响。
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