肉孢子虫属的分离、培养及冷冻保存
Isolation, Culture and Cryopreservation of Sarcocystis species.
作者信息
Verma S K, Lindsay D S, Grigg M E, Dubey J P
机构信息
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland.
Department of Biomedical Science and Pathology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia.
出版信息
Curr Protoc Microbiol. 2017 May 16;45:20D.1.1-20D.1.27. doi: 10.1002/cpmc.32.
Abstract
More than 200 valid Sarcocystis species have been described in the parasitological literature. The developmental life cycle in the intermediate host and definitive host has only been described for a few species. Sarcocystis parasites are common pathogens infecting a wide range of animals, including humans, and this unit reviews the methods used for isolating infective stages of the parasite from both definitive and intermediate host(s), as well as methods used to initiate cultures from sporocysts and merozoites and for cryopreservation of various Sarcocystis spp. These methods are based on published reports and our experience with Sarcocystis species in cell culture over many years. The information presented is suitable for the efficient culture of many Sarcocystis species; however, some minor modifications may be needed based on the unique developmental patterns of some species. © 2017 by John Wiley & Sons, Inc.
摘要
寄生虫学文献中已描述了200多种有效的肉孢子虫物种。仅对少数物种描述了其在中间宿主和终末宿主中的发育生命周期。肉孢子虫寄生虫是感染包括人类在内的多种动物的常见病原体，本单元综述了从终末宿主和中间宿主中分离寄生虫感染阶段的方法，以及从孢子囊和裂殖子开始培养以及对各种肉孢子虫物种进行冷冻保存的方法。这些方法基于已发表的报告以及我们多年来在细胞培养中对肉孢子虫物种的经验。所提供的信息适用于许多肉孢子虫物种的高效培养；然而，根据某些物种独特的发育模式，可能需要进行一些小的修改。© 2017约翰威立父子公司版权所有。
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