Peris María Paz, Gracia María Jesús, Moreno Bernardino, Juan-Puente Paula, Morales Mariano, Serrano María, Manzano María Dolores, Halaihel Nabil, Badiola Juan, Castillo Juan Antonio
Department of Animal Pathology, Faculty of Veterinary Sciences, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Instituto Universitario de Investigación Mixto Agroalimentario de Aragón (IA2), 50013 Zaragoza, Spain.
Vet Sci. 2024 Feb 27;11(3):103. doi: 10.3390/vetsci11030103.
spp. are complex apicomplexan parasites that cause a substantial economic impact on livestock used for meat production. These parasites are present worldwide. Our study aimed to identify species affecting sheep meat in southern-central Spain and to evaluate the effectiveness of freezing for parasite inactivation. A total of 210 condemned samples of sheep meat were thoroughly assessed grossly and microscopically; the presence of macro- and microcysts was confirmed. The samples were then frozen at -20 °C for various time intervals (24, 48, 72, 96, 120, and 144 h) and compared with untreated samples. Bradyzoites were isolated through pepsin digestion for subsequent molecular analysis and viability assessment, employing trypan blue and double fluorescence staining techniques. Our measurements confirmed the presence of , , and in Spanish domestic sheep. Freezing for 96 to 144 h resulted in a significant reduction in parasite viability, with a robust correlation observed between the two staining methods. Both stains effectively measured the viability of , thereby promising future advances in meat safety.
某些物种是复杂的顶复门寄生虫，会对用于肉类生产的牲畜造成重大经济影响。这些寄生虫在全球范围内都有存在。我们的研究旨在识别影响西班牙中南部绵羊肉的物种，并评估冷冻对寄生虫灭活的有效性。总共对210份被拒收的绵羊肉样本进行了全面的肉眼和显微镜评估；确认存在大囊和微囊。然后将样本在-20°C下冷冻不同时间间隔（24、48、72、96、120和144小时），并与未处理的样本进行比较。通过胃蛋白酶消化分离缓殖子，以便随后采用台盼蓝和双重荧光染色技术进行分子分析和活力评估。我们的测量证实了西班牙家养绵羊中存在某些物种。冷冻96至144小时导致寄生虫活力显著降低，两种染色方法之间观察到强烈的相关性。两种染色剂都能有效地测量某些物种的活力，从而有望在肉类安全方面取得未来进展。
