Zwadlo-Klarwasser G, Drube S, Schmutzler W
Institute of Pharmacology, Medical Faculty, RWTH Aachen, FRG.
J Investig Allergol Clin Immunol. 1992 Mar-Apr;2(2):73-7.
Subsets of macrophages (BM 8-, la- and esterase-positive subtypes) in the spleen and the peritoneum of mice were affected differently by immunomodulators during the humoral immune response. I.p. application of inosine pranobex (INPX), as well as of its salt moiety (DIPPACBA) and its dimethylaminopropanol (DIP) and acetamidobenzoic acid (PACBA) components, increased the number of nonspecific esterase-bearing cells in the peritoneum on day 3. INPX and DIPPACBA stimulated the la+ macrophage on day 3, DIPPACBA the BM 8+ macrophage only on day 1 and DIP on day 5. In spleen, DIP and PACBA had marked effects on the la+ subset in addition to a marginal effect on the BM 8+ phenotype on day 1. DIPPACBA also influenced the BM 8+ macrophage slightly on day 1. In contrast, the microbial product OK-432 stimulated BM 8+ and la+ macrophages in spleen markedly on day 1, but only marginally on days 3 and 5. However, it exerted a strong effect on both subtypes in peritoneum on days 3 and 5. OK-432 was found to be without any influence on esterase-bearing macrophages. The results show that the heterogeneity of macrophages is not only represented by subset markers, but also by their susceptibility to immunomodulators in different organs and stages of the immune response.
在体液免疫反应期间，小鼠脾脏和腹膜中的巨噬细胞亚群（BM 8-、Ia-和酯酶阳性亚型）受免疫调节剂的影响各不相同。腹腔注射肌苷普拉诺贝（INPX）及其盐部分（二异丙基氨基苯甲酸酯，DIPPACBA）、二甲基氨基丙醇（DIP）和对乙酰氨基苯甲酸（PACBA），在第3天增加了腹膜中含非特异性酯酶细胞的数量。INPX和DIPPACBA在第3天刺激Ia+巨噬细胞，DIPPACBA仅在第1天刺激BM 8+巨噬细胞，DIP在第5天刺激BM 8+巨噬细胞。在脾脏中，DIP和PACBA除了在第1天对BM 8+表型有轻微影响外，对Ia+亚群有显著影响。DIPPACBA在第1天对BM 8+巨噬细胞也有轻微影响。相比之下，微生物产物OK-432在第1天显著刺激脾脏中的BM 8+和Ia+巨噬细胞，但在第3天和第5天仅有轻微刺激。然而，它在第3天和第5天对腹膜中的两种亚型都有强烈影响。发现OK-432对含酯酶巨噬细胞没有任何影响。结果表明，巨噬细胞的异质性不仅由亚群标志物体现，还由它们在免疫反应的不同器官和阶段对免疫调节剂的敏感性体现。
