Ye Sang Ho, Watanabe Junji, Iwasaki Yasuhiko, Ishihara Kazuhiko
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Biomaterials. 2003 Oct;24(23):4143-52. doi: 10.1016/s0142-9612(03)00296-5.
The ideal surface of an artificial blood purification membrane needs hemocompatibility and durability of high performance; it should not adsorb any proteins or cells but should still have high permeability in the desired range of solute size. To improve the anti-fouling property of cellulose acetate (CA) membranes, a CA membrane blended with poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (PMB30) was designed as a blood purification membrane. The polymer solutions for preparing the membrane were prepared using a solvent mixture composed of N, N-dimethylformamide, acetone, 2-propanol or water. The CA and CA/PMB30 blend membranes with an asymmetric and porous structure were prepared by a phase inversion process. The characteristics of the CA/PMB30 blend membrane, such as structural properties, mechanical properties, and solute permeability were examined with attention to changes in the preparation conditions of the membrane. The CA/PMB30 blend membrane had good water and solute permeability and a sharp molecular weight cut-off property. Moreover, the amount of proteins adsorbed on the CA/PMB30 blend membrane surface was less than that of the original CA membrane and a conventional polysulfone membrane. Adhesion and activation of platelets on the CA/PMB30 blend membrane were reduced compared with that on a CA membrane. In addition, the CA/PMB30 blend membrane showed good permselectivity and an antifouling property during a long time ultrafiltration experiment with protein solutions.
理想的人工血液净化膜表面需要具备高性能的血液相容性和耐久性；它不应吸附任何蛋白质或细胞，但在所需的溶质尺寸范围内仍应具有高渗透性。为了提高醋酸纤维素（CA）膜的抗污染性能，设计了一种与聚（2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）-共-甲基丙烯酸正丁酯（BMA））（PMB30）共混的CA膜作为血液净化膜。制备该膜的聚合物溶液是使用由N，N-二甲基甲酰胺、丙酮、2-丙醇或水组成的溶剂混合物制备的。通过相转化过程制备了具有不对称和多孔结构的CA膜和CA/PMB30共混膜。关注膜制备条件的变化，研究了CA/PMB30共混膜的特性，如结构性能、机械性能和溶质渗透性。CA/PMB30共混膜具有良好的水和溶质渗透性以及尖锐的截留分子量特性。此外，CA/PMB30共混膜表面吸附的蛋白质量少于原始CA膜和传统聚砜膜。与CA膜相比，血小板在CA/PMB30共混膜上的黏附和活化减少。此外，在蛋白质溶液的长时间超滤实验中，CA/PMB30共混膜表现出良好的选择渗透性和抗污染性能。
