Rao Kakuturu V N, He Yi-Xun, Kalyanasundaram Ramaswamy
Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, Illinois 61107, USA.
Clin Diagn Lab Immunol. 2003 Jul;10(4):536-41. doi: 10.1128/cdli.10.4.536-541.2003.
A cloning and expression system that allows display of proteins on the surface of filamentous phages was exploited to display a 28-kDa glutathione S-transferase (Sm28GST) antigen of the human parasite Schistosoma mansoni. The phage-displayed Sm28GST (pdGST) was immunoreactive and was recognized by immune sera, suggesting that the Sm28GST protein displayed on the surface of phages potentially maintains native conformation. Subsequent immunization studies showed that mice can develop high titers of antibodies against pdGST and do not require any additional adjuvant for immunization. Isotype analysis suggested that the pdGST immunization predominantly induced immunoglobulin G2b (IgG2b), IgG3, and IgM anti-GST antibodies in mice. Furthermore, the pdGST immunization was found to confer about 30% protection after a challenge infection with 100 cercariae of S. mansoni in BALB/c mice. These findings suggest that phage display is a simple, efficient, and promising tool to express candidate vaccine antigens for immunization against infectious agents.
一种能使蛋白质展示在丝状噬菌体表面的克隆和表达系统，被用于展示人类寄生虫曼氏血吸虫的一种28 kDa谷胱甘肽S-转移酶（Sm28GST）抗原。噬菌体展示的Sm28GST（pdGST）具有免疫反应性，能被免疫血清识别，这表明展示在噬菌体表面的Sm28GST蛋白可能保持天然构象。随后的免疫研究表明，小鼠能产生高滴度的抗pdGST抗体，且免疫时不需要任何额外的佐剂。同种型分析表明，pdGST免疫主要诱导小鼠产生免疫球蛋白G2b（IgG2b）、IgG3和IgM抗GST抗体。此外，在BALB/c小鼠经100条曼氏血吸虫尾蚴攻击感染后，发现pdGST免疫可提供约30%的保护作用。这些发现表明，噬菌体展示是一种用于表达针对感染因子免疫的候选疫苗抗原的简单、高效且有前景的工具。
