Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan; Institute of Medical Sciences, Tzu Chi University, Hualien 97004, Taiwan.
Department of Laboratory Medicine and Biotechnology, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan.
Acta Trop. 2021 Oct;222:106033. doi: 10.1016/j.actatropica.2021.106033. Epub 2021 Jul 3.
Sm28GST is one of the candidate antigens for Schistosoma mansoni vaccine. Already Sm28GST vaccine formulations have shown to be protective against S. mansoni infection. Currently, efforts have been put into finding an adjuvant to enhance the immunity induced by Sm28GST. In the present work, we investigated whether heat-killed Propionibacterium acnes can be served as a potential adjuvant for recombinant Sm28GST (rSm28GST) antigen. As the results showed, P. acnes successfully modulated the Th1 humoral immune response induced by rSm28GST. Stronger Th1 cytokines responses were also observed in mice immunized with P. acnes-adjuvanted rSm28GST. Immunization of mice with P. acnes-adjuvanted rSm28GST was able to reduce worm burden and hepatic egg burden by 54.20 and 73.61%. Reduced granuloma size and count, as well as improved liver histology, were seen in P. acnes-adjuvanted rSm28GST immunized mice. These data suggest that P. acnes may evoke a stronger rSm28GST-induced immune response, higher resistance to S. mansoni infection, and more profound protection against S. mansoni-induced liver damages.
Sm28GST 是曼氏血吸虫疫苗的候选抗原之一。Sm28GST 疫苗配方已被证明能有效预防曼氏血吸虫感染。目前，人们正在努力寻找佐剂来增强 Sm28GST 诱导的免疫应答。在本研究中，我们研究了是否可以使用热灭活痤疮丙酸杆菌作为重组 Sm28GST（rSm28GST）抗原的潜在佐剂。结果表明，痤疮丙酸杆菌成功地调节了 rSm28GST 诱导的 Th1 体液免疫应答。在接受痤疮丙酸杆菌佐剂 rSm28GST 免疫的小鼠中，观察到更强的 Th1 细胞因子反应。用痤疮丙酸杆菌佐剂 rSm28GST 免疫的小鼠能够将虫体负荷和肝内虫卵负荷分别减少 54.20%和 73.61%。在接受痤疮丙酸杆菌佐剂 rSm28GST 免疫的小鼠中，肉芽肿的大小和数量减少，肝组织学得到改善。这些数据表明，痤疮丙酸杆菌可能引发更强的 rSm28GST 诱导的免疫应答，对曼氏血吸虫感染具有更高的抵抗力，并对曼氏血吸虫感染引起的肝损伤提供更深刻的保护。
