胃肿瘤中的碳酸酐酶同工酶IX和XII
Carbonic anhydrase isozymes IX and XII in gastric tumors.
作者信息
Leppilampi Mari, Saarnio Juha, Karttunen Tuomo J, Kivelä Jyrki, Pastoreková Silvia, Pastorek Jaromir, Waheed Abdul, Sly William S, Parkkila Seppo
机构信息
Department of Clinical Chemistry, P.O.Box 5000, FIN-90014 University of Oulu, Finland.
出版信息
World J Gastroenterol. 2003 Jul;9(7):1398-403. doi: 10.3748/wjg.v9.i7.1398.
AIM
To systematically study the expression of carbonic anhydrase (CA) isozymes IX and XII in gastric tumors.
METHODS
We analyzed a representative series of specimens from non-neoplastic gastric mucosa and from various dysplastic and neoplastic gastric lesions for the expression of CA IX and XII. Immunohistochemical staining was performed using isozyme-specific antibodies and biotin-streptavidin complex method.
RESULTS
CA IX was highly expressed in the normal gastric mucosa and remained positive in many gastric tumors. In adenomas, CA IX expression significantly decreased towards the high grade dysplasia. However, the expression resumed back to the normal level in well differentiated adenocarcinomas, while it again declined in carcinomas with less differentiation. In comparison, CA XII showed no or weak immunoreaction in the normal gastric mucosa and was slightly increased in tumors.
CONCLUSION
These results demonstrate that CA IX expression is sustained in several types of gastric tumors. The variations observed in the CA IX levels support the concept that gastric adenomas and carcinomas are distinct entities and do not represent progressive steps of a single pathway.
目的
系统研究碳酸酐酶（CA）同工酶IX和XII在胃肿瘤中的表达。
方法
我们分析了一系列具有代表性的标本，包括非肿瘤性胃黏膜以及各种发育异常和肿瘤性胃病变，以检测CA IX和XII的表达。使用同工酶特异性抗体和生物素-链霉亲和素复合物法进行免疫组织化学染色。
结果
CA IX在正常胃黏膜中高表达，在许多胃肿瘤中仍呈阳性。在腺瘤中，CA IX表达向高级别发育异常显著降低。然而，在高分化腺癌中表达恢复到正常水平，而在低分化癌中再次下降。相比之下，CA XII在正常胃黏膜中无免疫反应或免疫反应较弱，在肿瘤中略有增加。
结论
这些结果表明，CA IX在几种类型的胃肿瘤中持续表达。CA IX水平的变化支持胃腺瘤和癌是不同实体的概念，并不代表单一途径的渐进步骤。
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